Popova V
Vet Med Nauki. 1976;13(10):91-5.
Investigated were a total of 92 samples of pasteurized cans of different batches. Six strains of Clostridium perfringens were isolated from a sample of pasteurized ham. To demonstrate a toxin and present its type differentiation a strain was investigated with the use of dermonecrotic test after Williams along with the cross toxin neutralization after the internationally accepted method, and the neutralization of the lecithinase activity after Williams. The use of native toxins at the type differentiation of slightly toxigenic strains of Clostridium perfringens isolated from food products proved inappropriate. The production of dry toxin and the application of cross toxinneutralization proved to be most suitable in the typing of such strains. The type differentiation carried out as described above showed that the studied strain belonged to type A.
共调查了92份不同批次的巴氏杀菌罐头样本。从一份巴氏杀菌火腿样本中分离出6株产气荚膜梭菌。为了证明毒素并进行类型区分,采用威廉姆斯氏皮肤坏死试验以及国际公认方法进行交叉毒素中和试验,并采用威廉姆斯氏法对卵磷脂酶活性进行中和试验,对一株菌株进行了研究。在从食品中分离出的轻度产毒产气荚膜梭菌菌株的类型区分中,使用天然毒素被证明是不合适的。干燥毒素的产生以及交叉毒素中和试验的应用被证明是对此类菌株进行分型的最合适方法。按上述方法进行的类型区分表明,所研究的菌株属于A型。