Kavvadias Dominique, Scherer Gerhard, Urban Michael, Cheung Francis, Errington Graham, Shepperd Jim, McEwan Mike
Analytisch-biologisches Forschungslabor GmbH, Goethestrasse 20, 80336 Munich, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Apr 15;877(11-12):1185-92. doi: 10.1016/j.jchromb.2009.03.009. Epub 2009 Mar 16.
Tobacco-specific N-nitrosamines (TSNA) include 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), N-nitrosoanabasine (NAB) and N-nitrosoanatabine (NAT). TSNA are suggested to play an important role in tobacco smoke carcinogenesis. We have developed and validated an LC-MS/MS method for the determination of total (free and conjugated) TSNA in human urine. The limits of detection (LOD) were 2.0, 0.8, 1.1 and 0.7 pg/ml for NNAL, NNN, NAB and NAT, respectively. Smokers were found to have significantly higher levels of TSNA in their urine than nonsmokers. In conclusion, the newly developed method is suitable for assessing the tobacco use-related exposure to NNK, NNN, NAB and NAT.
烟草特有亚硝胺（TSNA）包括4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁酮（NNK）、N-亚硝基降烟碱（NNN）、N-亚硝基新烟草碱（NAB）和N-亚硝基假木贼碱（NAT）。TSNA被认为在烟草烟雾致癌过程中起重要作用。我们开发并验证了一种用于测定人尿中总（游离和结合）TSNA的液相色谱-串联质谱法。对于NNAL、NNN、NAB和NAT，检测限（LOD）分别为2.0、0.8、1.1和0.7 pg/ml。发现吸烟者尿液中TSNA水平显著高于不吸烟者。总之，新开发的方法适用于评估与烟草使用相关的NNK、NNN、NAB和NAT暴露情况。
