Pu Lee L Q, Cui Xiangdong, Fink Betsy F, Cibull Michael L, Gao Dayong
University of Kentucky, Lexington, KY, USA.
Aesthet Surg J. 2005 Mar-Apr;25(2):126-31. doi: 10.1016/j.asj.2005.01.003.
Several studies have shown that one of the sugars, trehalose, can improve tissue survival after cryopreservation when combined with other cryoprotective agents, and thus may possibly be used in cryopreservation of adipose tissues that have been found more resistant to injury after freezing.
The purpose of this study was to test our hypothesis that lipoplasty-derived adipose aspirates could be effectively cryopreserved by adding trehalose as the sole cryoprotective agent (CPA), and to develop a practical technique to effectively preserve adipose tissues for future applications.
The middle layer of adipose aspirates obtained from conventional lipoplasty was collected after centrifugation and each specimen was randomized into 3 groups: the control group, fresh adipose aspirates without preservation; the simple cryopreservation group (no CPA); and the optimal cryopreservation group (with trehalose as a CPA). Cryopreservation of adipose aspirates was conducted with controlled slow cooling and fast rewarming rates. Fresh or cryopreserved adipose aspirates in each group were evaluated by viable adipocyte counts, glycerol-3-phosphate dehydrogenase (G3PDH) assay, and routine histology.
More viable adipocytes and better cellular function of adipose aspirates were found in the optimal cryopreservation group compared to the simple cryopreservation group, but these results were less ideal than results from the control group.
An optimal cryopreservation method using trehalose as a CPA appears to provide better long-term preservation of adipose aspirates than a simple cryopreservation method. Further studies are needed to refine our method for cryopreservation with trehalose as a CPA and confirm our findings in vivo.
多项研究表明，糖类之一的海藻糖与其他冷冻保护剂联合使用时，可提高冷冻保存后的组织存活率，因此可能用于冷冻保存已发现对冷冻损伤更具抵抗力的脂肪组织。
本研究旨在验证我们的假设，即通过添加海藻糖作为唯一的冷冻保护剂（CPA），可有效冷冻保存抽脂术获得的脂肪抽吸物，并开发一种切实可行的技术来有效保存脂肪组织以供未来应用。
将常规抽脂术获得的脂肪抽吸物的中间层离心后收集，每个样本随机分为3组：对照组，未保存的新鲜脂肪抽吸物；单纯冷冻保存组（无CPA）；最佳冷冻保存组（以海藻糖作为CPA）。采用控制缓慢冷却和快速复温速率对脂肪抽吸物进行冷冻保存。通过活脂肪细胞计数、甘油-3-磷酸脱氢酶（G3PDH）测定和常规组织学评估每组新鲜或冷冻保存的脂肪抽吸物。
与单纯冷冻保存组相比，最佳冷冻保存组的脂肪抽吸物中有更多的活脂肪细胞和更好的细胞功能，但这些结果不如对照组理想。
使用海藻糖作为CPA的最佳冷冻保存方法似乎比单纯冷冻保存方法能更好地长期保存脂肪抽吸物。需要进一步研究以完善我们以海藻糖作为CPA的冷冻保存方法，并在体内证实我们的发现。
