采用校准的自动化血栓图检测法检测血浆中的内源性组织因子水平。
Detection of endogenous tissue factor levels in plasma using the calibrated automated thrombogram assay.
机构信息
Inserm, U698, Paris, F-75018 France, Université Paris 7, Paris, F-75018 France.
出版信息
Thromb Res. 2010 Jan;125(1):90-6. doi: 10.1016/j.thromres.2009.03.003. Epub 2009 Apr 5.
BACKGROUND
The calibrated automated thrombogram (CAT) assay measures thrombin generation in plasma.
OBJECTIVE
Use the CAT assay to detect endogenous tissue factor (TF) in recalcified platelet-rich plasma (PRP) and platelet-free plasma (PFP).
METHODS
Blood from healthy volunteers was collected into citrate and incubated at 37 degrees C with or without lipopolysaccharide (LPS) for 5 hours. PRP and PFP were prepared and clotting was initiated by recalcification. Thrombin generation was measured using the CAT assay.
RESULTS
The lag time (LT) was significantly shortened in PRP prepared from LPS-treated blood compared with untreated blood (10+/-3 min versus 20+/-6 min), and this change was reversed by the addition of inactivated human factor VIIa. LPS stimulation did not change the peak thrombin. Similar results were observed in PFP (21+/-4 min versus 35+/-5 min). LPS stimulation also significantly reduced the LT of PRP and PFP derived from blood containing citrate and a factor XIIa inhibitor. Finally, a low concentration of exogenous TF shortened the LT of PFP prepared from unstimulated, citrated blood without affecting the peak thrombin.
CONCLUSION
Changes in LT in the CAT assay can be used to monitor levels of endogenous TF in citrated plasma.
背景
校准的自动化血栓图（CAT）测定法可测量血浆中的凝血酶生成。
目的
使用 CAT 测定法检测复钙血小板富血浆（PRP）和无血小板血浆（PFP）中的内源性组织因子（TF）。
方法
从健康志愿者采集柠檬酸血，并在 37°C 下孵育，有或没有脂多糖（LPS）孵育 5 小时。制备 PRP 和 PFP，并通过复钙引发凝血。使用 CAT 测定法测量凝血酶生成。
结果
与未处理的血液相比，来自 LPS 处理的血液的 PRP 的迟滞时间（LT）明显缩短（10+/-3 分钟对 20+/-6 分钟），并且这种变化可被失活的人因子 VIIa 逆转。LPS 刺激不会改变最大凝血酶。在 PFP 中也观察到类似的结果（21+/-4 分钟对 35+/-5 分钟）。LPS 刺激还显著缩短了源自含有柠檬酸和因子 XIIa 抑制剂的血液的 PRP 和 PFP 的 LT。最后，低浓度的外源性 TF 缩短了未刺激的、柠檬酸化的血液中 PFP 的 LT，而不影响最大凝血酶。
结论
CAT 测定法中的 LT 变化可用于监测柠檬酸血浆中内源性 TF 的水平。
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