Hao X L, Kuang H, Li Y L, Yuan Y, Peng C F, Chen W, Wang L B, Xu C L
School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214000, China.
J Agric Food Chem. 2009 Apr 22;57(8):3033-9. doi: 10.1021/jf803807b.
A general and broad class selective competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the alpha-cyano pyrethroids was developed. One class specific hapten, the 5-(3-benzylphenyl)-5-cyanopentanoic acid (CPBA-BE), was conjugated with BSA as an immunogen. Polyclonal antibodies, PY-antibodies, were generated. The assay with the most selectivity for the family alpha-cyano pyrethroids such as cyphenothrin was further optimized and tested for tolerance to cosolvent, pH, and ionic strength changes. The IC(50) values of the optimized immunoassay were 4.58 microg L(-1) for cyphenothrin, 5.62 microg L(-1) for fenpropathrin, 7.08 microg L(-1) for deltamethrin, 10.72 microg L(-1) for cypermethrin, 19.95 microg L(-1) for flucythrinate, and 28.18 microg L(-1) for esfenvalerate. The cross-reactivities of cyphenothrin, fenpropathrin, deltamethrin, cypermethrin, flucythrinate, and esfenvalerate were 100%, 81.49%, 64.68%, 42.72%, 22.96%, and 16.25%, respectively. The low detection limit (LDL) of this assay was 0.107 microg L(-1) for cyphenothrin. This method was used for the detection of the alpha-cyano pyrethroids in Tai lake water.
开发了一种用于检测α-氰基拟除虫菊酯的通用且宽泛的类选择性竞争性酶联免疫吸附测定法（ELISA）。一种类特异性半抗原，即5-（3-苄基苯基）-5-氰基戊酸（CPBA-BE），与牛血清白蛋白（BSA）偶联作为免疫原。制备了多克隆抗体，即PY抗体。对该测定法进行了进一步优化，使其对诸如苯醚菊酯等α-氰基拟除虫菊酯类具有最高选择性，并测试了其对助溶剂、pH值和离子强度变化的耐受性。优化后的免疫测定法对苯醚菊酯的半数抑制浓度（IC50）值为4.58 μg L-1，甲氰菊酯为5.62 μg L-1，溴氰菊酯为7.08 μg L-1，氯氰菊酯为10.72 μg L-1，氟氰戊菊酯为19.95 μg L-1，高效氯氟氰菊酯为28.18 μg L-1。苯醚菊酯、甲氰菊酯、溴氰菊酯、氯氰菊酯、氟氰戊菊酯和高效氯氟氰菊酯的交叉反应率分别为100%、81.49%、64.68%、42.72%、22.96%和16.25%。该测定法对苯醚菊酯的最低检测限（LDL）为0.107 μg L-1。该方法用于检测太湖水体中的α-氰基拟除虫菊酯。
