Kilpatrick David R, Yang Chen-Fu, Ching Karen, Vincent Annelet, Iber Jane, Campagnoli Ray, Mandelbaum Mark, De Lina, Yang Su-Ju, Nix Allan, Kew Olen M
Polio and Picornavirus Laboratory Branch, G-10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
J Clin Microbiol. 2009 Jun;47(6):1939-41. doi: 10.1128/JCM.00702-09. Epub 2009 Apr 22.
We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.
我们已将之前描述的脊髓灰质炎病毒诊断性逆转录聚合酶链反应(RT-PCR)检测方法改编为实时RT-PCR(rRT-PCR)形式。我们高度特异的检测方法和rRT-PCR试剂专为世界卫生组织全球脊髓灰质炎实验室网络设计,用于快速大规模鉴定脊髓灰质炎病毒野外分离株。