针对神经突生长抑制剂进行DNA疫苗接种以促进创伤性脑损伤后的功能恢复。

DNA vaccination against neurite growth inhibitors to enhance functional recovery following traumatic brain injury.

作者信息

Zhang Yi, Ang Beng Ti, Xiao Zhi Cheng, Ng Ivan

机构信息

Department of Neurosurgery, National Neuroscience Institute, 11 Jalan Tan Tock Seng, Tan Tock Seng, Singapore 308433.

出版信息

Acta Neurochir Suppl. 2008;102:347-51. doi: 10.1007/978-3-211-85578-2_66.

DOI:10.1007/978-3-211-85578-2_66

PMID:19388343

Abstract

BACKGROUND

Myelin-associated proteins contribute to failure of axon regeneration in the injured central nervous system of the adult.

METHODS

In this study, we employed a recombinant DNA vaccine encoding the myelin-derived inhibitors NogoA, myelin-associated glycoprotein (MAG) and tenascin-R (TnR), so as to effect the production of antibodies against these myelin-related antigens, in a rodent head injury model and ascertained its potential for promoting axonal plasticity and functional recovery. Adult rats underwent lateral fluid percussion at the left sensorimotor cortex (SMC) and treatment with the DNA vaccine before or after injury. Behavioral tests and neuroanatomical tract tracing was carried out.

FINDINGS

The vaccinated rats showed improved corticorubral plasticity and functional recovery compared to control groups.

CONCLUSIONS

This suggests that a DNA vaccination approach may provide a promising strategy for promoting repair after traumatic brain injury.

摘要

背景

髓磷脂相关蛋白导致成体受伤中枢神经系统中轴突再生失败。

方法

在本研究中，我们采用了一种重组DNA疫苗，其编码髓磷脂衍生抑制剂NogoA、髓磷脂相关糖蛋白（MAG）和腱生蛋白-R（TnR），以便在啮齿动物头部损伤模型中产生针对这些髓磷脂相关抗原的抗体，并确定其促进轴突可塑性和功能恢复的潜力。成年大鼠在左侧感觉运动皮层（SMC）接受侧方流体冲击伤，并在损伤前后用DNA疫苗进行治疗。进行了行为测试和神经解剖学追踪。