Kline Kelli G, Finney Greg L, Wu Christine C
NRSA, Wu laboratory.
Brief Funct Genomic Proteomic. 2009 Mar;8(2):114-25. doi: 10.1093/bfgp/elp008.
The ultimate goal of most shotgun proteomic pipelines is the discovery of novel biomarkers to direct the development of quantitative diagnostics for the detection and treatment of disease. Differential comparisons of biological samples identify candidate peptides that can serve as proxys of candidate proteins. While these discovery approaches are robust and fairly comprehensive, they have relatively low throughput. When merged with targeted mass spectrometry, this pipeline can fuel hypothesis-driven studies and the development of novel diagnostics and therapeutics.
大多数鸟枪法蛋白质组学流程的最终目标是发现新型生物标志物,以指导用于疾病检测和治疗的定量诊断方法的开发。对生物样品进行差异比较可识别出能够作为候选蛋白质替代物的候选肽段。虽然这些发现方法强大且相当全面,但通量相对较低。当与靶向质谱联用后,该流程可为基于假设的研究以及新型诊断和治疗方法的开发提供助力。