人巨噬细胞对继发登革病毒感染患者血小板的吞噬作用增强。

Increased phagocytosis of platelets from patients with secondary dengue virus infection by human macrophages.

作者信息

Honda Shoko, Saito Mariko, Dimaano Efren M, Morales Philip A, Alonzo Maria T G, Suarez Lady-Anne C, Koike Natsuki, Inoue Shingo, Kumatori Atsushi, Matias Ronald R, Natividad Filipinas F, Oishi Kazunori

机构信息

Department of Clinical Medicine and Virology, Institute of Tropical Medicine Nagasaki University, Japan.

出版信息

Am J Trop Med Hyg. 2009 May;80(5):841-5.

PMID:19407135

Abstract

The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and the peripheral platelet counts as well as platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections. The percent phagocytosis and the levels of PAIgG were significantly increased in these patients during the acute phase compared with the healthy volunteers. The increased percent phagocytosis and PAIgG found during the acute phase significantly decreased during the convalescent phase. An inverse correlation between platelet count and the percent phagocytosis (P = 0.011) and the levels of PAIgG (P = 0.041) was found among these patients during the acute phase. No correlation was found, however, between the percent phagocytosis and the levels of PAIgG. Our present data suggest that accelerated platelet phagocytosis occurs during the acute phase of secondary DV infections, and it is one of the mechanisms of thrombocytopenia in this disease.

摘要

利用流式细胞术检测了36例继发登革病毒（DV）感染患者中分化的THP-1细胞对血小板的吞噬百分比，并检测了外周血小板计数以及血小板相关IgG（PAIgG）。与健康志愿者相比，这些患者急性期的吞噬百分比和PAIgG水平显著升高。急性期发现的吞噬百分比增加和PAIgG在恢复期显著降低。在这些患者的急性期，血小板计数与吞噬百分比（P = 0.011）和PAIgG水平（P = 0.041）之间存在负相关。然而，吞噬百分比与PAIgG水平之间未发现相关性。我们目前的数据表明，继发DV感染的急性期会加速血小板吞噬，这是该疾病血小板减少的机制之一。