组织芯片评估腺瘤性息肉病 coli、β-连环蛋白、E-钙黏蛋白和细胞周期蛋白 D1 在食管鳞状细胞癌中的表达及意义

Expression and significance of adenomatous polyposis coli, beta-catenin, E-cadherin and cyclin D1 in esophageal squamous cell carcinoma assessed by tissue microarray.

作者信息

Peng Hui, Zhong Xue-Yun, Liu Kun-Ping, Li Su-Mei

机构信息

Department of Pathology, Medical School, Jinan University, Guangzhou, Guangdong, PR China.

出版信息

Ai Zheng. 2009 Jan;28(1):38-41. Epub 2009 Jan 10.

PMID:19448414

Abstract

BACKGROUND AND OBJECTIVE

The genesis of esophageal squamous cell carcinoma(ESCC)is a multifactor and multistage process, in which Wnt signaling transduction pathway plays an important role in tumorigenesis and tumor progression. This study was to investigate the roles of four proteins in the Wnt pathway in tumorigenesis of ESCC, and their significances in the early diagnosis of ESCC.

METHODS

The expression of adenomatous polyposis coli (APC), beta-catenin, E-cadherin and cyclinD1 was detected by immunohistochemistry using tissue microarrays consisting of 199 specimens of ESCC, 164 specimens of normal mucosa, 34 specimens of basal cell hyperplasia and 30 specimens of dysplasia adjacent to cancer tissues.

RESULTS

The positive rates of APC and E-cadherin in ESCC were lower than those in the normal group (69.6% vs. 98.0%, p < 0.01; 19.6% vs. 96.3%, p < 0.01). The abnormal expression rates of beta-catenin and cyclin D1 in ESCC were higher than those in the normal group (65.5% vs. 1.2%,p < 0.01; 70.9% vs. 0.8%, p < 0.01). In accordance with the following order, normal epithelia --> basal cell hyperplasia --> dysplasia --> ESCC, hypoexpression of APC proteins occurred in ESCC, abnormalities of beta-catenin and E-cadherin started to appear in dysplasia, and overexpression of Cyclin D1 emerged from basal cell hyperplasia. From well to poorly differentiated ESCC, the expression of APC, E-cadherin and cyclin D1 were gradually reduced, while beta-catenin was increased. The expression of beta-catenin was not correlated with APC (r = -0.10, p > 0.05), was negatively correlated with E-cadherin (r = -0.31,p < 0.01) and positively correlated with cyclin D1(r = 0.49, p < 0.01).

CONCLUSION

APC, E-cadherin, beta-catenin and cyclin D1 may play important roles in tumorigenesis of ESCC. Therefore, detection of E-cadherin, beta-catenin and cyclin D1 proteins may be helpful for the early diagnosis of ESCC.

摘要

背景与目的

食管鳞状细胞癌（ESCC）的发生是一个多因素、多阶段的过程，其中Wnt信号转导通路在肿瘤发生和发展中起重要作用。本研究旨在探讨Wnt通路中四种蛋白在ESCC肿瘤发生中的作用及其在ESCC早期诊断中的意义。

方法

采用免疫组织化学法检测199例ESCC标本、164例正常黏膜标本、34例基底细胞增生标本和30例癌旁不典型增生标本组成的组织芯片中腺瘤性息肉病 coli（APC）、β-连环蛋白、E-钙黏蛋白和细胞周期蛋白D1的表达。

结果

ESCC中APC和E-钙黏蛋白的阳性率低于正常组（69.6%对98.0%，p<0.01；19.6%对96.3%，p<0.01）。ESCC中β-连环蛋白和细胞周期蛋白D1的异常表达率高于正常组（65.5%对1.2%，p<0.01；70.9%对0.8%，p<0.01）。按照正常上皮→基底细胞增生→不典型增生→ESCC的顺序，ESCC中APC蛋白表达降低，β-连环蛋白和E-钙黏蛋白的异常在不典型增生时开始出现，细胞周期蛋白D1的过表达从基底细胞增生时开始出现。从高分化到低分化ESCC，APC、E-钙黏蛋白和细胞周期蛋白D1的表达逐渐降低，而β-连环蛋白增加。β-连环蛋白的表达与APC无相关性（r=-0.10，p>0.05），与E-钙黏蛋白呈负相关（r=-0.31，p<0.01），与细胞周期蛋白D1呈正相关（r=0.49，p<0.01）。