Spåhr Henrik, Calero Guillermo, Bushnell David A, Kornberg Roger D
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9185-90. doi: 10.1073/pnas.0903361106. Epub 2009 May 20.
The second structure of a eukaryotic RNA polymerase II so far determined, that of the enzyme from the fission yeast Schizosaccharomyces pombe, is reported here. Comparison with the previous structure of the enzyme from the budding yeast Saccharomyces cerevisiae reveals differences in regions implicated in start site selection and transcription factor interaction. These aspects of the transcription mechanism differ between S. pombe and S. cerevisiae, but are conserved between S. pombe and humans. Amino acid changes apparently responsible for the structural differences are also conserved between S. pombe and humans, suggesting that the S. pombe structure may be a good surrogate for that of the human enzyme.
本文报道了迄今为止所确定的真核生物RNA聚合酶II的第二种结构,即来自裂殖酵母粟酒裂殖酵母的该酶的结构。与来自芽殖酵母酿酒酵母的该酶先前结构进行比较,发现在与起始位点选择和转录因子相互作用相关的区域存在差异。转录机制的这些方面在粟酒裂殖酵母和酿酒酵母之间有所不同,但在粟酒裂殖酵母和人类之间是保守的。显然导致结构差异的氨基酸变化在粟酒裂殖酵母和人类之间也是保守的,这表明粟酒裂殖酵母的结构可能是人类该酶结构的良好替代物。