离子液体对反相高效液相色谱法中2-氯苯酚和2,4,6-三氯苯酚分离的影响

Effects of ionic liquid on the separation of 2-chlorophenol and 2,4,6-trichlorophenol in RP-HPLC.

作者信息

Zheng J, Row K H

机构信息

Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, Incheon 402-751, South Korea.

出版信息

J Chromatogr Sci. 2009 May-Jun;47(5):392-5. doi: 10.1093/chromsci/47.5.392.

DOI:10.1093/chromsci/47.5.392

PMID:19476708

Abstract

In this study, the use of ionic liquids as additives in the separation of 2-chlorophenol and 2,4,6-trichlorophenol in reversed-phase high-performance liquid chromatography is reported. The ionic liquids used were 1-butyl-3-methylimidazolium tetrafluoroborate, 1-ethyl-3-methylimidazolium tetrafluoroborate, 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]), and 1-octyl-3-methylimidazolium methylsulfate ([OMIm][MS]), because of their high hydrophobicity and good solvating properties. The effects of the ionic liquid on retention factor and resolution, and the change of detector response using the different ionic liquids were studied. Separation with the [MS] anion ionic liquid in the eluent resulted in better resolution than with the [BF4] anion ionic liquid. The best result was achieved using 3.0 mM/L of [OMIm][MS] and 1.0 mM/L [EMIm][MS] as mobile phase additives.

摘要

本研究报道了在反相高效液相色谱中使用离子液体作为添加剂分离2-氯苯酚和2,4,6-三氯苯酚。所使用的离子液体为1-丁基-3-甲基咪唑四氟硼酸盐、1-乙基-3-甲基咪唑四氟硼酸盐、1-乙基-3-甲基咪唑甲基硫酸盐（[EMIm][MS]）和1-辛基-3-甲基咪唑甲基硫酸盐（[OMIm][MS]），因其具有高疏水性和良好的溶剂化性能。研究了离子液体对保留因子和分离度的影响，以及使用不同离子液体时检测器响应的变化。在洗脱液中使用含[MS]阴离子的离子液体进行分离，其分离度优于含[BF4]阴离子的离子液体。以3.0 mM/L的[OMIm][MS]和1.0 mM/L的[EMIm][MS]作为流动相添加剂时，获得了最佳结果。