丙泊酚对大鼠脑出血损伤的保护作用。

Protective effect of propofol against intracerebral hemorrhage injury in rats.

作者信息

Ma Jie, Dong Zhi, Li Qin-geng, Wang Jun-rui

机构信息

Key Laboratory of Biochemistry and Molecular Pharmacology, Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China.

出版信息

Yao Xue Xue Bao. 2009 Apr;44(4):344-9.

PMID:19545049

Abstract

The neuroprotective effect of propofol against intracerebral hemorrhage (ICH) in rats was investigated. ICH was induced in rats by infusion of collagenase (Type VII) 0.5 U (1 U x microL(-1)) into the left caudate nucleus. Three doses of propofol were given intraperitoneally (i.p.) 10 min before collagenase infusion. Effects of propofol on neurological behavioral scores, brain water content (BWC), activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in brain tissue, expression level of caspase-3 were studied. In propofol groups (30 and 100 mg x kg(-1)), the neurological behavioral score, BWC and the content of MDA were significantly lower than those in ICH group (P < 0.05, P < 0.01), whereas the activity of SOD was higher than that in ICH group (P < 0.05). Meanwhile, propofol (15, 30, and 100 mg x kg(-1)) inhibited caspase-3 expression in dose-dependent manner (r = 0.877). Brain damages caused by ICH in rats can be alleviated by propofol, which mechanism might be attributed to its antioxidant activity.

摘要

研究了丙泊酚对大鼠脑出血（ICH）的神经保护作用。通过向大鼠左侧尾状核注射0.5 U（1 U×μL⁻¹）VII型胶原酶诱导ICH。在注射胶原酶前10分钟腹腔注射（i.p.）三种剂量的丙泊酚。研究了丙泊酚对神经行为评分、脑含水量（BWC）、脑组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量、半胱天冬酶-3表达水平的影响。在丙泊酚组（30和100 mg×kg⁻¹）中，神经行为评分、BWC和MDA含量显著低于ICH组（P < 0.05，P < 0.01），而SOD活性高于ICH组（P < 0.05）。同时，丙泊酚（15、30和100 mg×kg⁻¹）以剂量依赖性方式抑制半胱天冬酶-3表达（r = 0.877）。丙泊酚可减轻大鼠ICH引起的脑损伤，其机制可能归因于其抗氧化活性。