一种在中国养殖兔中流行的新型戊型肝炎病毒基因型。

A novel genotype of hepatitis E virus prevalent among farmed rabbits in China.

作者信息

Zhao Chenyan, Ma Zhongren, Harrison Tim J, Feng Ruofei, Zhang Chuntao, Qiao Zilin, Fan Jinping, Ma Hongxia, Li Mingsheng, Song Aijing, Wang Youchun

机构信息

Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China.

出版信息

J Med Virol. 2009 Aug;81(8):1371-9. doi: 10.1002/jmv.21536.

DOI:10.1002/jmv.21536

PMID:19551838

Abstract

In total, 335 serum samples were collected from rabbits from two farms in Gansu province, China, and tested for anti-hepatitis E virus (HEV) antibody using EIA and for HEV RNA using nested RT- PCR with ORF2 primers. The overall prevalence of anti-HEV antibody and HEV RNA was 57.0% (191/335) and 7.5% (25/335), respectively. The positivity rate of HEV RNA in the anti-HEV antibody negative group (7.6% (11/144)) did not differ significantly from that in the positive group (7.3% (14/191)). The concordance between HEV RNA and anti-HEV antibody was 43.3% with no significant correlation (P < 0.05). All 25 amplicons from the ORF2 region were cloned and sequenced. On the basis of nucleotide sequence comparison, they had 84-99% identity to each other and 73-77%, 70-76%, 75-82%, 71-77%, and 53-65% with the corresponding regions of genotypes 1, 2, 3, 4, and avian HEV, respectively. Samples that were positive with the ORF2 primers were amplified using ORF1 region primers; 17 were positive and shared 71-78%, 73-76%, 74-82%, 72-78%, and 39-58% identity with the corresponding regions of genotypes 1, 2, 3, 4, and avian HEV, respectively, at the nucleotide level. Two representative full-length sequences were determined. These two sequences shared 85% identity with each other and had 74%, 73%, 78-79%, 74-75%, and 46-47% identity to full-length genotypes 1, 2, 3, 4, and avian HEV, respectively. Thus, the sequences isolated from the rabbits represent a novel genotype of HEV. This study provides novel information about HEV genotypes infecting rabbits as well as evidence of a new mammalian genotype of HEV.

摘要

总共从中国甘肃省的两个养殖场的兔子中采集了335份血清样本，使用酶免疫分析（EIA）检测抗戊型肝炎病毒（HEV）抗体，并使用针对开放阅读框2（ORF2）引物的巢式逆转录聚合酶链反应（RT-PCR）检测HEV RNA。抗HEV抗体和HEV RNA的总体流行率分别为57.0%（191/335）和7.5%（25/335）。抗HEV抗体阴性组中HEV RNA的阳性率（7.6%（11/144））与阳性组（7.3%（14/191））相比无显著差异。HEV RNA与抗HEV抗体之间的一致性为43.3%，无显著相关性（P<0.05）。对来自ORF2区域的所有25个扩增子进行了克隆和测序。基于核苷酸序列比较，它们彼此之间的同源性为84%-99%，与1、2、3、4型基因型以及禽HEV相应区域的同源性分别为73%-77%、70%-76%、75%-82%、71%-77%和53%-65%。使用ORF1区域引物对ORF2引物检测呈阳性的样本进行扩增；17个样本呈阳性，在核苷酸水平上与1、2、3、4型基因型以及禽HEV相应区域的同源性分别为71%-78%、73%-76%、74%-82%、72%-78%和39%-58%。确定了两个代表性的全长序列。这两个序列彼此之间的同源性为85%，与全长1、2、3、4型基因型以及禽HEV的同源性分别为74%、73%、78%-79%、74%-75%和46%-47%。因此从兔子中分离出的序列代表了一种新基因型的HEV。本研究提供了关于感染兔子的HEV基因型的新信息以及一种新的哺乳动物HEV基因型的证据。