高尔基体后囊泡的胞吐作用受内吞机制成分的调节。
Exocytosis of post-Golgi vesicles is regulated by components of the endocytic machinery.
作者信息
Jaiswal Jyoti K, Rivera Victor M, Simon Sanford M
机构信息
The Rockefeller University, 1230 York Avenue, Box 304, New York, NY 10065, USA.
出版信息
Cell. 2009 Jun 26;137(7):1308-19. doi: 10.1016/j.cell.2009.04.064.
Post-Golgi vesicles target and deliver most biosynthetic cargoes to the cell surface. However, the molecules and mechanisms involved in fusion of these vesicles are not well understood. We have employed a system to simultaneously monitor release of luminal and membrane biosynthetic cargoes from individual post-Golgi vesicles. Exocytosis of these vesicles is not calcium triggered. Release of luminal cargo can be accompanied by complete, partial, or no release of membrane cargo. Partial and no release of membrane cargo of a fusing vesicle are fates associated with kiss-and-run exocytosis, and we find that these are the predominant mode of post-Golgi vesicle exocytosis. Partial cargo release by post-Golgi vesicles occurs because of premature closure of the fusion pore and is modulated by the activity of clathrin, actin, and dynamin. Our results demonstrate that these components of the endocytic machinery modulate the nature and extent of biosynthetic cargo delivery by post-Golgi vesicles at the cell membrane.
高尔基体后囊泡将大多数生物合成货物靶向运输并递送至细胞表面。然而，这些囊泡融合过程中涉及的分子和机制尚未完全明晰。我们采用了一种系统来同时监测单个高尔基体后囊泡中腔内和膜生物合成货物的释放。这些囊泡的胞吐作用并非由钙触发。腔内货物的释放可能伴随着膜货物的完全释放、部分释放或不释放。融合囊泡的膜货物部分释放和不释放是与吻-跑式胞吐作用相关的命运，并且我们发现这些是高尔基体后囊泡胞吐作用的主要模式。高尔基体后囊泡的部分货物释放是由于融合孔过早关闭所致，并受到网格蛋白、肌动蛋白和发动蛋白活性的调节。我们的结果表明，内吞机制的这些组分调节了高尔基体后囊泡在细胞膜处进行生物合成货物递送的性质和程度。
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