[采用中心组合设计-响应面法对万寿菊总黄酮最佳提取工艺的研究]
[Study on the optimal extaction of total flavonoids from Tagetes erecta by central composite design-response surface methodology].
作者信息
Jia Chang-Ping, Tian Bao-Cheng, Li Yan-Bing, Gao Ying-Bo, Yu Bo-Meng, Pan Wei-Ling
机构信息
Heilongjiang University of Chinese Medicine, Harbin 150040, China.
出版信息
Zhong Yao Cai. 2009 Mar;32(3):430-2.
OBJECTIVE
To optimize the process of extracting total flavonoids from Tagetes erecta.
METHODS
The influential factors were extraction temperature, ethanol concentration, reflux time and solvent volume fold. The evaluating indicator was the extraction rate of total flavonoids from Tagetes erecta. The central composite design-response surface methodology was used to optimize the process and the prediction was carried out.
RESULTS
The optimum conditions of extraction were 80% ethanol, 2.5 hours for reflux, 35 volume folds of solvent and 70 degrees C.
CONCLUSION
It shows that the optimum model is simple and highly predictive.
目的
优化万寿菊中总黄酮的提取工艺。
方法
考察提取温度、乙醇浓度、回流时间和溶剂体积倍数等影响因素。以万寿菊总黄酮提取率为评价指标。采用中心复合设计-响应面法对工艺进行优化并进行预测。
结果
最佳提取条件为80%乙醇、回流2.5小时、溶剂体积倍数为35倍、温度70℃。
结论
结果表明最佳模型简单且预测性强。
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