Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università Federico II, 80131 Napoli, Italy.
Int J Med Microbiol. 2009 Dec;299(8):535-46. doi: 10.1016/j.ijmm.2009.05.004. Epub 2009 Jul 1.
The whole DNA sequences of 2 Stenotrophomonas maltophilia strains isolated from the blood of a cancer patient (K279a) and the poplar Populus trichocarpa (R551-3) have been compared. The 2 chromosomes exhibit extensive synteny, but each is punctuated by about 40 genomic islands (GEIs), which vary in size from 3 to 70kb, and may encode up to about 50 proteins. A large set of smaller DNA sequences, encoding strain-specific 'solo' orfs, contributes to genetic heterogeneity in a significant manner. S. maltophilia GEIs potentially encode several proteins mediating interactions with the environment such as transmembrane proteins, haemagglutinins, components of type I and IV secretion systems, and efflux proteins having a role in metal and/or drug resistance. The presence of specific GEIs in the S. maltophilia population was monitored by PCR and slot-blot analyses. Data suggest that some islands are present at sites different from those identified in K279a and that alternative islands may be integrated at mapped sites.
已比较了从癌症患者血液（K279a）和杨树（Populus trichocarpa）（R551-3）中分离出的 2 株嗜麦芽寡养单胞菌菌株的全 DNA 序列。这 2 条染色体表现出广泛的同线性，但每条染色体都被约 40 个基因组岛（GEI）打断，大小从 3 到 70kb 不等，可能编码多达约 50 种蛋白质。一组较大的较小 DNA 序列，编码菌株特异性的“独奏”ORF，以显著方式促成遗传异质性。嗜麦芽寡养单胞菌 GEI 可能编码几种介导与环境相互作用的蛋白质，如跨膜蛋白、血凝集素、I 型和 IV 型分泌系统的组成部分以及在金属和/或药物抗性中起作用的外排蛋白。通过 PCR 和槽式印迹分析监测嗜麦芽寡养单胞菌种群中特定 GEI 的存在。数据表明，一些岛存在于与 K279a 中鉴定的位点不同的位置，并且替代岛可能在映射的位点处整合。
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