Scancar Janez, Milacic Radmila
Department of Environmental Sciences, Jozef Stefan Institute, Ljubljana, Slovenia.
J Sep Sci. 2009 Aug;32(15-16):2495-503. doi: 10.1002/jssc.200900219.
Liquid chromatography combined with element specific detection is commonly applied in speciation analysis. In these analyses, to obtain reliable data chemical species should not be transformed. To preserve chemical species during the separation step, fast chromatographic procedures and mild separation conditions are required. Monolithic supports that enable rapid chromatographic separations have rarely been used in speciation analysis. Methacrylate-based anion- and cation-exchange monolithic supports offer separation of charged chemical species of elements and can be used as a complementary tool to particle-packed liquid chromatographic columns. The present paper presents an overview of successful applications of methacrylate-based monolithic supports in speciation of zinc (Zn), chromium (Cr), and aluminium (Al) in environmental, occupational health, and biological samples. Measures of analytical performance of convective interaction media (CIM) monolithic chromatographic supports, namely selectivity, sensitivity, and time of analysis, are compared to those of particle-packed columns. The potential of CIM monolithic chromatography in speciation analysis is critically discussed. Direct comparison of the experimental data in speciation of elements by ion-exchange monolithic and fast protein liquid chromatography is reported for the first time. Finally, some recommendations are given for further investigations of the potential of monolithic chromatography and its implementations in different fields of element speciation analysis.
液相色谱结合元素特异性检测常用于形态分析。在这些分析中，为获得可靠数据，化学形态不应发生转化。为在分离步骤中保留化学形态，需要快速的色谱程序和温和的分离条件。能够实现快速色谱分离的整体式载体在形态分析中很少被使用。基于甲基丙烯酸酯的阴离子和阳离子交换整体式载体可用于分离元素的带电化学形态，并且可作为填充颗粒液相色谱柱的补充工具。本文概述了基于甲基丙烯酸酯的整体式载体在环境、职业健康和生物样品中锌（Zn）、铬（Cr）和铝（Al）形态分析中的成功应用。将对流相互作用介质（CIM）整体式色谱载体的分析性能指标，即选择性、灵敏度和分析时间，与填充颗粒柱的指标进行了比较。对CIM整体式色谱在形态分析中的潜力进行了批判性讨论。首次报道了通过离子交换整体式色谱和快速蛋白质液相色谱对元素形态分析实验数据的直接比较。最后，针对进一步研究整体式色谱的潜力及其在元素形态分析不同领域的应用给出了一些建议。
