决明子生品与炒制品的高效液相色谱指纹图谱比较
[Comparison on the HPLC fingerprint of Cassia obtusifolia between the raw and roasted seeds].
作者信息
Li Guiliu, Xiao Yongqing, Zhang Cun, Li Li, Pang Zhen
机构信息
Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Mar;34(6):694-7.
OBJECTIVE
To establish HPLC fingerprint for the identification of Cassia obtusifolia and compare chemical constituents of the raw and roasted seeds of C. obtusifolia.
METHOD
Chromatographic fingerprint of C. obtusifolia was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation. Data were analyzed by fingerprint similarity evaluation software to compare the similarity of the samples for identifying the main chromatographic peaks furthermore.
RESULT
Having compared the HPLC fingerprint of the raw and roasted seeds of C. obtusifolia were compared preliminarily, and 12 main chromatographic peaks were identified.
CONCLUSION
HPLC method can be used in quality control and identification of the raw and roasted seeds of C. obtusifolia.
目的
建立决明子的高效液相色谱指纹图谱用于决明子的鉴别，并比较决明子生品与炒品的化学成分。
方法
采用反相高效液相色谱法研究决明子的色谱指纹图谱，色谱分离采用梯度洗脱模式。运用指纹图谱相似度评价软件对数据进行分析，比较样品的相似度以进一步确定主要色谱峰。
结果
对决明子生品与炒品的高效液相色谱指纹图谱进行了初步比较，确定了12个主要色谱峰。
结论
高效液相色谱法可用于决明子生品与炒品的质量控制和鉴别。
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