来自红藻多指塞布藻的硫酸化木甘露聚糖：结构特征、化学修饰及抗病毒活性。

Sulfated xylomannans from the red seaweed Sebdenia polydactyla: structural features, chemical modification and antiviral activity.

作者信息

Ghosh Tuhin, Pujol Carlos Alberto, Damonte Elsa Beatriz, Sinha Sharmistha, Ray Bimalendu

机构信息

Natural Products Laboratory, Department of Chemistry, University of Burdwan, West Bengal, India.

出版信息

Antivir Chem Chemother. 2009;19(6):235-42. doi: 10.1177/095632020901900603.

DOI:10.1177/095632020901900603

PMID:19641232

Abstract

BACKGROUND

Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy.

METHODS

In this study, we analysed polysaccharide fractions isolated from Sebdenia polydactyla.

RESULTS

The purified xylomannan sulfate and its further sulfated derivatives showed strong activity against herpes simplex virus type-1 (HSV-1). Their 50% inhibitory concentration values were in the range 0.35-2.8 microg/ml and they lacked cytotoxicity at concentrations up to 1,000 microg/ml. The major polysaccharide, which had 0.6 sulfate groups per monomer unit and an apparent molecular mass of 150 kDa, contained a backbone of alpha-(1-->3)-linked d-mannopyranosyl residues substituted at position 6 with a single stub of beta-d-xylopyranosyl residues.

CONCLUSIONS

The degree of sulfation seemed to play an important role because desulfation and/or further sulfation of the isolated macromolecules largely influenced their in vitro anti-HSV-1 activity.

摘要

背景

许多病毒对细胞表面硫酸乙酰肝素蛋白聚糖具有亲和力，这在病毒进入过程中具有生物学相关性。这增加了硫酸化多糖在抗病毒治疗中应用的可能性。

方法

在本研究中，我们分析了从多趾海棉（Sebdenia polydactyla）中分离得到的多糖组分。

结果

纯化的硫酸木聚甘露糖及其进一步硫酸化的衍生物对1型单纯疱疹病毒（HSV-1）显示出强大的活性。它们的50%抑制浓度值在0.35 - 2.8微克/毫升范围内，并且在高达1000微克/毫升的浓度下没有细胞毒性。主要多糖每个单体单元含有0.6个硫酸基团，表观分子量为150 kDa，其主链由α-(1→3)-连接的D-甘露吡喃糖基残基组成，在第6位被单个β-D-木吡喃糖基残基短链取代。