在塑料板上使用多重聚合酶链反应（PCR）结合引物延伸对转基因大豆和玉米的特定基因进行光学检测。

Optical detection of specific genes for genetically modified soybean and maize using multiplex PCR coupled with primer extension on a plastic plate.

作者信息

Harikai Naoki, Saito Shin, Abe Midori, Kondo Kazunari, Kitta Kazumi, Akiyama Hiroshi, Teshima Reiko, Kinoshita Kenji

机构信息

School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.

出版信息

Biosci Biotechnol Biochem. 2009 Aug;73(8):1886-9. doi: 10.1271/bbb.90215. Epub 2009 Aug 7.

DOI:10.1271/bbb.90215

PMID:19661710

Abstract

A novel DNA microarray method to detect one line of genetically modified (GM) soybean and five lines of GM maize was developed using multiplex PCR coupled with primer extension on a plastic plate. Multiplex PCR products were applied on an extension primer-immobilized plate and the spots corresponding to the DNA sequences were visualized. This method is a rapid and simple way to detect GM soybean and GM maize optically.

摘要

一种新型的DNA微阵列方法被开发出来，该方法利用多重聚合酶链反应（PCR）结合塑料板上的引物延伸技术，用于检测一行转基因大豆和五行转基因玉米。多重PCR产物被应用于固定有延伸引物的板上，对应于DNA序列的斑点得以可视化。这种方法是一种快速且简单的光学检测转基因大豆和转基因玉米的方式。