从郁金香鳞茎中纯化和鉴定一种郁金香苷转化酶
Purification and characterization of a tuliposide-converting enzyme from bulbs of Tulipa gesneriana.
作者信息
Kato Yasuo, Shoji Kazuaki, Ubukata Makoto, Shigetomi Kengo, Sato Yukio, Nakajima Noriyuki, Ogita Shinjiro
机构信息
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Japan.
出版信息
Biosci Biotechnol Biochem. 2009 Aug;73(8):1895-7. doi: 10.1271/bbb.90226. Epub 2009 Aug 7.
An enzyme that catalyzes the stoichiometric conversion of 6-tuliposide into tulipalin was purified and characterized from bulbs of Tulipa gesneriana. The enzyme appeared to be a dimer, the relative molecular mass (Mr) of each subunit being 34,900; it had maximum activity and stability at neutral pH and moderate temperature. The enzyme preferentially acted on such glucose esters as 6-tuliposides, and to a lesser extent on p-nitrophenylacetate.
从郁金香的球茎中纯化并鉴定出一种能催化6-郁金香苷化学计量转化为郁金香素的酶。该酶似乎是一种二聚体，每个亚基的相对分子质量（Mr）为34,900；它在中性pH和适中温度下具有最大活性和稳定性。该酶优先作用于6-郁金香苷等葡萄糖酯，对乙酸对硝基苯酯的作用程度较小。
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