果蝇14-3-3ζ基因编码了第三种在蘑菇体神经元中富集的功能性异构体。
A third functional isoform enriched in mushroom body neurons is encoded by the Drosophila 14-3-3zeta gene.
作者信息
Messaritou Georgia, Leptourgidou Flora, Franco Maribel, Skoulakis Efthimios M C
机构信息
Institute of Molecular Biology and Genetics, Biomedical Sciences Research Centre Alexander Fleming, Vari, Greece.
出版信息
FEBS Lett. 2009 Sep 3;583(17):2934-8. doi: 10.1016/j.febslet.2009.08.003. Epub 2009 Aug 6.
14-3-3 proteins are highly conserved across eukaryotes, typically encoded by multiple genes in most species. Drosophila has only two such genes, 14-3-3zeta (leo), encoding two isoforms LEOI and LEOII, and 14-3-3epsilon. We report a bona fide third functional isoform encoded by leo divergent from the other two in structurally and functionally significant areas, thus increasing 14-3-3 diversity in Drosophila. Furthermore, we used a novel approach of spatially restricted leo abrogation by RNA-interference and revealed differential LEO distribution in adult heads, with LEOIII enrichment in neurons essential for learning and memory in Drosophila.
14-3-3蛋白在真核生物中高度保守，在大多数物种中通常由多个基因编码。果蝇只有两个这样的基因，即14-3-3ζ（leo），编码两种异构体LEOI和LEOII，以及14-3-3ε。我们报道了一种由leo编码的真正的第三种功能性异构体，它在结构和功能的重要区域与其他两种不同，从而增加了果蝇中14-3-3的多样性。此外，我们使用了一种通过RNA干扰在空间上限制leo消除的新方法，并揭示了LEO在成年果蝇头部的差异分布，其中LEOIII在果蝇学习和记忆所必需的神经元中富集。
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