[呼吸窘迫综合征合并呼吸机相关性肺炎新生儿支气管肺泡灌洗术中白细胞介素-4和白细胞介素-13浓度]

[Interleukin-4 and interleukin-13 concentrations in bronchoalveolar lavage fluid in neonates with respiratory distress syndrome and concurrent ventilator-associated pneumonia].

作者信息

Xia Yao-Fang, Liu Cui-Qing, Shi Hui-Ju, Ma Li

机构信息

Department of Neonatology, Hebei Province Children's Hospital, Shijiazhuang, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2009 Aug;11(8):645-8.

PMID:19695190

Abstract

OBJECTIVE

To study the concentrations of IL-4 and IL-13 in bronchoalveolar lavage fluid (BALF) in neonates with respiratory distress syndrome (RDS) and concurrent ventilator-associated pneumonia (VAP).

METHODS

Sixty-eight neonates with RDS undergoing mechanical ventilation for over 48 hrs were enrolled. IL-4 and IL-13 levels in BALF were measured using ELISA 1, 72 and 96 hrs after mechanical ventilation. The results were compared between the neonates with concurrent VAP (n=37) and without (n=31).

RESULTS

The levels of BALF IL-4 96 hrs after ventilation in the VAP group (35.34+/-1.78 ng/mL) were significantly higher than those in the non-VAP group (13.69+/-2.47 ng/mL, P<0.05). The levels of BALF IL-13 96 hrs after ventilation in the VAP group (33.74+/-2.74 ng/mL) also increased significantly compared with those in the non-VAP group (13.50+/-3.81 ng/mL) (P<0.05). There were significant differences in BALF IL-4 and IL-13 levels between 1 hr and 96 hrs in the VAP group (P<0.05).

CONCLUSIONS

BALF IL-4 and IL-13 levels increase in neonates with RDS and concurrent VAP. IL-4 and IL-13 may involve in the regulation of the inflammatory immune response.

摘要

目的

研究呼吸窘迫综合征（RDS）合并呼吸机相关性肺炎（VAP）新生儿支气管肺泡灌洗液（BALF）中白细胞介素-4（IL-4）和白细胞介素-13（IL-13）的浓度。

方法

纳入68例机械通气超过48小时的RDS新生儿。在机械通气后1、72和96小时，采用酶联免疫吸附测定法（ELISA）检测BALF中IL-4和IL-13水平。比较合并VAP的新生儿（n=37）和未合并VAP的新生儿（n=31）的检测结果。

结果

VAP组通气96小时后BALF中IL-4水平（35.34±1.78 ng/mL）显著高于非VAP组（13.69±2.47 ng/mL，P<0.05）。VAP组通气96小时后BALF中IL-13水平（33.74±2.74 ng/mL）也较非VAP组（13.50±3.81 ng/mL）显著升高（P<0.05）。VAP组BALF中IL-4和IL-13水平在1小时和96小时之间存在显著差异（P<0.05）。