Department of Otorhinolaryngology, School of Medicine, Mersin University, Ihsaniye mahallesi 123. cad. 4931 sokak, 33079 Mersin, Turkey.
Eur Arch Otorhinolaryngol. 2010 Feb;267(2):227-32. doi: 10.1007/s00405-009-1066-9. Epub 2009 Aug 23.
There is an ongoing dilemma about the pathogenesis of nasal polyp (NP). The etiology of NP is multifactorial. Reactive oxygen species and oxidative stress are also suggested to be among the possible factors in NP development. Glutathione-S-transferase (GST) is one of the important detoxifying enzymes. It is not known whether GST plays any role in NP development. We aimed to investigate the relationship between GST subgroup (GSTT1, GSTM1, and GSTP1) polymorphisms, and NP development. Seventy-five patients with NP with or without asthma (NP with asthma: 22, NP without asthma: 53) were used as a study group. As much as 167 healthy individuals were involved as the control group. NP diagnosis was made by nasal endoscopy and paranasal sinus computed tomography (CT). NP was defined as the presence of endoscopically visible bilateral polyps originated from the middle meatus to the nasal cavity and affecting more than one paranasal sinus confirmed by CT. Blood was collected in EDTA-containing tubes and DNA was extracted from the leukocytes. The genotyping of polymorphisms of GSTT1, GSTM1, and GSTP1 were done using real time polymerase chain reaction. Chi-square (chi(2)) and Fisher's (F) exact tests were used for statistical evaluation. A 2-fold increased risk of NP could be found in individuals with the GSTT1 null genotype (OR = 2.03, 95% CI = 1.03-4.011). The distribution of GSTM1 null genotypes was not significantly different between the NP patients and controls and there was also no significance between the GSTP1 genotypes and NP. In conclusion, GST gene polymorphisms may be important in pathogenesis of NP. Additional studies which include larger study groups in different geographic localizations may be more useful to evaluate association with GST polymorphism and NP.
鼻息肉（NP）的发病机制一直存在争议。NP 的病因是多因素的。活性氧和氧化应激也被认为是 NP 发展的可能因素之一。谷胱甘肽-S-转移酶（GST）是重要的解毒酶之一。目前尚不清楚 GST 是否在 NP 发展中发挥作用。我们旨在研究 GST 亚群（GSTT1、GSTM1 和 GSTP1）多态性与 NP 发展之间的关系。75 例 NP 患者（伴或不伴哮喘，NP 伴哮喘：22 例，NP 不伴哮喘：53 例）作为研究组。167 名健康个体作为对照组。NP 的诊断依据为鼻内窥镜和鼻窦计算机断层扫描（CT）。NP 定义为内窥镜下可见双侧息肉，从中鼻甲到鼻腔起源，影响一个以上鼻窦，CT 证实。采集含 EDTA 的管中的血液，并从白细胞中提取 DNA。使用实时聚合酶链反应对 GSTT1、GSTM1 和 GSTP1 多态性的基因分型进行检测。采用卡方（chi(2)）和 Fisher（F）确切检验进行统计学评价。具有 GSTT1 缺失基因型的个体 NP 的风险增加 2 倍（OR = 2.03，95%CI = 1.03-4.011）。NP 患者和对照组之间 GSTM1 缺失基因型的分布无显著差异，GSTP1 基因型与 NP 之间也无显著差异。结论：GST 基因多态性可能在 NP 的发病机制中起重要作用。在不同地理位置包括更大的研究组的进一步研究可能更有助于评估 GST 多态性与 NP 的相关性。
