A data base for polymer chromatography: temperature dependence of interaction parameters in liquid adsorption chromatography.

The temperature dependence of retention behaviour of polyethylene glycol (PEG) and its mono- and dimethyl ethers was studied on various RP columns in different mobile phases. The accessible volumes and the interaction parameters were determined from slope and intercept in a plot of the elution volumes of the oligomers of a polymer homologous series as a function of the difference of the elution volumes of consecutive oligomers. A quite different dependence of the interaction parameters was observed in the different mobile phases. While in methanol-water the interaction parameter decreases with increasing temperature, the opposite effect is observed in acetonitrile (ACN)-water. In acetone-water, the temperature dependence is almost negligible.