来自酿酒酵母的端粒酶在体外作为单体具有活性。

Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer.

作者信息

Shcherbakova D M, Sokolov K A, Zvereva M I, Dontsova O A

机构信息

Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia.

出版信息

Biochemistry (Mosc). 2009 Jul;74(7):749-55. doi: 10.1134/s0006297909070074.

DOI:10.1134/s0006297909070074

PMID:19747095

Abstract

A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. Our results indicate that yeast telomerase is active in vitro as a monomer.

摘要

开发了一种通过TLC1 RNA中的RNA亲和标签分离酵母端粒酶的系统。链霉亲和素适体被插入到TLC1 RNA的两个不同位置。带有这些插入片段之一的TLC1 RNA的端粒酶在体内具有功能，并且可以在体外通过亲和层析进行分离。使用该技术从产生两种可区分的TLC1 RNA分子（有和没有适体插入）的菌株中分离得到的端粒酶制剂，结果分离出仅含有带有适体的TLC1 RNA的活性端粒酶。我们的结果表明酵母端粒酶在体外作为单体具有活性。