三种新型绵羊基因-SLC25A4、SLC25A5 和 SLC25A6 的分离、核苷酸鉴定和组织表达。
Isolation, nucleotide identification and tissue expression of three novel ovine genes-SLC25A4, SLC25A5 and SLC25A6.
机构信息
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
出版信息
Mol Biol Rep. 2010 Jul;37(6):2743-8. doi: 10.1007/s11033-009-9812-z. Epub 2009 Sep 11.
The complete coding sequences of three of sheep genes SLC25A4, SLC25A5 and SLC25A6 were firstly amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) according to the conserved sequence information of the cattle or other mammals and known highly homologous sheep ESTs. Sheep SLC25A4, SLC25A5 and SLC25A6 genes encode three corresponding proteins of 298 amino acids which contain the identically conserved putative mitochondrial carrier protein domain. Sheep SLC25A4 protein has high homology with the SLC25A4 proteins of six species-cattle (99%), human (95%), rat (95%), mouse (94%), dog (94%) and chicken (89%). Sheep SLC25A5 protein has high identity with the SLC25A5 proteins of five species-cattle (100%), dog (99%), mouse (98%), rat (98%) and human (98%). Sheep SLC25A6 protein also has high homology with the SLC25A6 proteins of four species-cattle (99%), human (97%), pig (97%) and chicken (93%). The phylogenetic tree analysis demonstrated that sheep SLC25A4, SLC25A5 and SLC25A6 proteins share a common ancestor. Moreover, SLC25A4, SLC25A5 and SLC25A6 proteins present stronger interaction each other. The tissue expression analysis indicated that sheep SLC25A4, SLC25A5 and SLC25A6 genes were expressed in a range of tissues including leg muscle, kidney, skin, longissimus dorsi muscle, spleen, heart and liver. Our experiment is the first to provide the primary foundation for further insight into these three sheep genes.
首先根据牛或其他哺乳动物的保守序列信息和已知的高度同源绵羊 EST，使用逆转录聚合酶链反应（RT-PCR）扩增了绵羊基因 SLC25A4、SLC25A5 和 SLC25A6 的完整编码序列。绵羊 SLC25A4、SLC25A5 和 SLC25A6 基因编码三个对应的 298 个氨基酸的蛋白质，它们包含相同保守的假定线粒体载体蛋白结构域。绵羊 SLC25A4 蛋白与六种物种（牛 99%、人 95%、大鼠 95%、小鼠 94%、狗 94%和鸡 89%）的 SLC25A4 蛋白具有高度同源性。绵羊 SLC25A5 蛋白与五种物种（牛 100%、狗 99%、小鼠 98%、大鼠 98%和人 98%）的 SLC25A5 蛋白具有高度同一性。绵羊 SLC25A6 蛋白也与四种物种（牛 99%、人 97%、猪 97%和鸡 93%）的 SLC25A6 蛋白具有高度同源性。系统进化树分析表明，绵羊 SLC25A4、SLC25A5 和 SLC25A6 蛋白具有共同的祖先。此外，SLC25A4、SLC25A5 和 SLC25A6 蛋白之间存在较强的相互作用。组织表达分析表明，绵羊 SLC25A4、SLC25A5 和 SLC25A6 基因在多种组织中表达，包括腿部肌肉、肾脏、皮肤、背最长肌、脾脏、心脏和肝脏。我们的实验首次为进一步深入研究这三个绵羊基因提供了初步基础。
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