[YPK1过表达导致酿酒酵母对盐胁迫超敏感依赖于TOR1]
[YPK1 overexpression resulted salt stress hypersensitivity in Saccharomyces cerevisiae is dependent on TOR1].
作者信息
Cao Hongping, Wang Yihui, Zhong Yan, Liu Xin, Miao Ming, Duan Wei, Liu Ke
机构信息
Key Laboratory of Bio-resource and Eco-environment, Ministry of Education, School of Life Science, Sichuan University, Chengdu 610064, China.
出版信息
Wei Sheng Wu Xue Bao. 2009 Aug;49(8):1069-74.
UNLABELLED
Serine/threonine protein kinase YPK1, a homologue of mammalian protein kinase serum and glucocorticoid-inducible kinase (SGK), plays important physiological roles in Saccharomyces cerevisiae. It involves in cell wall maintenance, actin cytoskeleton dynamics, endocytosis, translation during nitrogen starvation and nutrient sensing.
OBJECTIVE
In order to further explore the cellular function of YPK1 and the its transduction pathway.
METHODS
We overexpressed YPK1 in wild type and tor1 mutant cells and monitored the cell growth responding to salt stress.
RESULTS
We found that YPK1 overexpression resulted salt stress hypersensitivity in wild type cells and this hypersensitivity was abolished by tor1 mutant.
CONCLUSION
Our results indicate that YPK1 may interact with target of rapamycin (TOR1) to regulate the salt stress response.
未标记
丝氨酸/苏氨酸蛋白激酶YPK1是哺乳动物蛋白激酶血清和糖皮质激素诱导激酶（SGK）的同源物，在酿酒酵母中发挥重要的生理作用。它参与细胞壁维持、肌动蛋白细胞骨架动力学、内吞作用、氮饥饿期间的翻译以及营养感知。
目的
为了进一步探索YPK1的细胞功能及其转导途径。
方法
我们在野生型和tor1突变体细胞中过表达YPK1，并监测细胞对盐胁迫的生长反应。
结果
我们发现YPK1过表达导致野生型细胞对盐胁迫超敏，而tor1突变消除了这种超敏性。
结论
我们的结果表明YPK1可能与雷帕霉素靶蛋白（TOR1）相互作用以调节盐胁迫反应。
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