Park Sung-Yong, Kalim Sheraz, Callahan Caitlin, Teitell Michael A, Chiou Eric P Y
Department of Mechanical and Aerospace Engineering, University of California at Los Angeles (UCLA), 43-147 Eng. IV, 420 Westwood Plaza, Los Angeles, CA 90095-1597, USA.
Lab Chip. 2009 Nov 21;9(22):3228-35. doi: 10.1039/b909158k. Epub 2009 Sep 10.
We report on a light-actuated, droplet based microfluidic platform enabling two-dimensional (2D) droplet manipulation on an open chamber with a single-side, featureless photoconductive surface. The droplet actuation mechanism is based on recently demonstrated floating electrode optoelectronic tweezers (FEOET), which enable light-induced dielectrophoretic forces to manipulate aqueous droplets immersed in electrically nonconductive oil, with a light intensity as low as 400 microW/cm2. In this paper, we study the shape effect of optical patterns for 2D droplet actuation, and demonstrate light-actuated droplet manipulation functions including 2D droplet transport, merging, mixing, and multidroplet processing, for up to 16 droplets in parallel. Such an open chamber platform also permits easy interfacing and integration with other microfluidic structures, such as wells and close-channel based droplet devices to increase its versatility for biochemical analyses.
我们报道了一种基于液滴的光驱动微流控平台，该平台能够在具有单侧无特征光电导表面的开放腔室中进行二维（2D）液滴操控。液滴驱动机制基于最近展示的浮动电极光电镊子（FEOET），它能够利用光诱导的介电泳力来操控浸没在非导电油中的水滴，所需光强度低至400微瓦/平方厘米。在本文中，我们研究了用于二维液滴驱动的光学图案的形状效应，并展示了光驱动液滴操控功能，包括二维液滴传输、合并、混合以及多达16个液滴的并行多液滴处理。这种开放腔室平台还便于与其他微流控结构（如孔和基于封闭通道的液滴装置）进行接口连接和集成，以增加其在生化分析中的通用性。
