冷冻显微镜法测定“结合水”。
THE CRYOSCOPIC METHOD FOR THE DETERMINATION OF "BOUND WATER".
机构信息
Division of Agricultural Biochemistry, The University of Minnesota, St. Paul.
出版信息
J Gen Physiol. 1934 Jan 20;17(3):327-39. doi: 10.1085/jgp.17.3.327.
Abstract
- The criticisms by Grollman (7) of the cryoscopic method for the determination of bound water as proposed by Newton and Gortner (1) have been considered, and it is pointed out that even admitting the correctness of his contentions does not negative the conclusion that bound water values as determined by the cryoscopic method parallel in a remarkable manner the physiological responses of plants to environmental conditions. 2. A new method of calculating the true freezing point of a solution is proposed. 3. Gum acacia in aqueous sucrose solutions shows positive amounts of bound water to the extent of 0.6 to 0.7 gm. of bound water per gram of gum. 4. Gum acacia in aqueous solutions of KCl and KBr shows slightly negative amounts of bound water, indicating a preferential adsorption of the solute rather than the solvent.
摘要
格罗曼（7）对牛顿和戈特纳（1）提出的冰点法测定结合水的批评已经被考虑在内，并且指出，即使承认他的论点是正确的，也不能否定这样的结论，即冰点法测定的结合水值与植物对环境条件的生理反应非常相似。
提出了一种新的计算溶液真实冰点的方法。
在含有蔗糖的水溶液中，阿拉伯胶表现出正的结合水量，达到每克胶 0.6 至 0.7 克的结合水。
在 KCl 和 KBr 的水溶液中，阿拉伯胶显示出略微的负结合水量，表明溶质的优先吸附而不是溶剂。
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