Sachon Emmanuelle, Matheron Lucrèce, Clodic Gilles, Blasco Thierry, Bolbach Gérard
UPMC, 7-9 quai Saint Bernard, bâtiment A, Plateforme de Protéomique et spectrométrie de masse, IFR83, 75005 Paris, France.
J Mass Spectrom. 2010 Jan;45(1):43-50. doi: 10.1002/jms.1687.
Disposable plasticware such as plastic test tubes are routinely used in all proteomics laboratories. Additives in polymers are used to protect them against oxygen or ultraviolet (UV) light degradation. Hindered amine light stabilizers (HALSs) are of utmost importance in modern polyolefin (polypropylene, polyethylene) stabilization. In this article, we demonstrate that the manufacturing polymeric agent: poly-(N-beta-hydroxyethyl-2,2,6,6-tetramethyl-4-hydroxy-piperidinyl succinate), known as Tinuvin-622 or Lowilite 62, from the HALS family, leaches from laboratory polypropylene or polyethylene plastic test tubes into the standard solvents for sample preparation. The analysis of these polluted samples by matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry, in the positive mode, shows highly contaminated mass spectra, due to the high sensitivity of this technique. These contaminants have mass range and mass defect similar to those of peptides arising from the digestion of a protein in a conventional proteomics study. Therefore, they can be really harmful for proteomics studies, leading to misattributions, preventing any protein identification. In this article, an MS and MS/MS fingerprint of this pollutant is given and some pieces of advice to avoid it are proposed.
一次性塑料制品，如塑料试管，在所有蛋白质组学实验室中都经常使用。聚合物中的添加剂用于保护它们免受氧气或紫外线（UV）光降解。受阻胺光稳定剂（HALSs）在现代聚烯烃（聚丙烯、聚乙烯）稳定化中至关重要。在本文中，我们证明了制造聚合物试剂：聚-（N-β-羟乙基-2,2,6,6-四甲基-4-羟基哌啶基琥珀酸酯），即来自HALS家族的Tinuvin-622或Lowilite 62，会从实验室聚丙烯或聚乙烯塑料试管中沥出到用于样品制备的标准溶剂中。通过基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱在正模式下对这些污染样品进行分析，由于该技术的高灵敏度，显示出高度污染的质谱图。这些污染物的质量范围和质量缺陷与传统蛋白质组学研究中蛋白质消化产生的肽相似。因此，它们对蛋白质组学研究可能非常有害，导致错误归因，阻碍任何蛋白质鉴定。本文给出了这种污染物的质谱和串联质谱指纹图谱，并提出了一些避免它的建议。
