缬草水提物对杏仁核点燃大鼠的抗惊厥作用：可能涉及腺苷。
Anticonvulsant effect of aqueous extract of Valeriana officinalis in amygdala-kindled rats: possible involvement of adenosine.
机构信息
Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
出版信息
J Ethnopharmacol. 2010 Feb 3;127(2):313-8. doi: 10.1016/j.jep.2009.11.002. Epub 2009 Nov 10.
ETHNOPHARMACOLOGICAL RELEVANCE
Valeriana officinalis L. (valerian) root extract has been used as an antiepileptic herbal medicine in Iran.
AIM OF THIS STUDY
In the present study the effect of valerian extracts on an experimental model of temporal lobe epilepsy (TLE) was evaluated. Moreover, the involvement of adenosine system in the actions of aqueous extract of valerian was evaluated.
MATERIALS AND METHODS
Bipolar stimulating and monopolar recording electrodes were implanted stereotaxically in the right basolateral amygdala of male Sprague-Dawley rats. After kindling, the effect of aqueous (200, 500 and 800 mg/kg; intraperitoneal) and petroleum ether (PE; 50 and 100mg/kg; intraperitoneal) extracts of valerian and CPT (selective A(1) receptor antagonist; 10 and 20 microM; intracerebroventricular) on afterdischarge duration (ADD), duration of stage 5 seizure (S5D) and latency to the onset of bilateral forelimb clonuses (S4L) were measured. The effect of CPT (10 microM) on the response of aqueous extract of valerian (500 mg/kg) was also determined.
RESULTS
The results showed that aqueous extract of valerian had anticonvulsant effect. However, PE extract and CPT (20 microM) had proconvulsant effect. Administration of CPT (10 microM) before the administration of aqueous extract decreased the anticonvulsant effect of valerian.
CONCLUSIONS
The results showed significant anticonvulsant effect for aqueous but not PE extract of valerian. Moreover, CPT as a selective adenosine A(1) receptor antagonist decreased the anticonvulsant effect of valerian aqueous extract. Therefore, we concluded that part of anticonvulsant effect of valerian probably is mediated through activation of adenosine system.
民族药理学相关性
缬草根提取物已在伊朗被用作抗癫痫草药。
研究目的
本研究评估缬草提取物对颞叶癫痫（TLE）实验模型的影响。此外，还评估了腺嘌呤系统在缬草水提取物作用中的参与情况。
材料和方法
双极刺激和单极记录电极通过立体定向植入雄性 Sprague-Dawley 大鼠右侧基底外侧杏仁核。在点燃后，评估缬草水（200、500 和 800mg/kg；腹腔内）和石油醚（PE；50 和 100mg/kg；腹腔内）提取物以及 CPT（选择性 A1 受体拮抗剂；10 和 20μM；脑室内）对后放电持续时间（ADD）、5 期癫痫发作持续时间（S5D）和双侧前肢强直发作潜伏期（S4L）的影响。还确定了 CPT（10μM）对缬草水提取物（500mg/kg）的反应的影响。
结果
结果表明，缬草水提取物具有抗惊厥作用。然而，PE 提取物和 CPT（20μM）具有促惊厥作用。在给予缬草水提取物之前给予 CPT（10μM）降低了缬草的抗惊厥作用。
结论
结果表明，缬草的水提取物具有显著的抗惊厥作用，但 PE 提取物没有。此外，作为选择性腺嘌呤 A1 受体拮抗剂的 CPT 降低了缬草水提取物的抗惊厥作用。因此，我们得出结论，缬草的部分抗惊厥作用可能是通过激活腺嘌呤系统介导的。
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