Molecular Biology Division, Veterans Affairs Medical Center, San Francisco, CA 94121, USA.
FEBS Lett. 2010 Jan 4;584(1):159-65. doi: 10.1016/j.febslet.2009.11.022.
Alkaline incubation of NADH results in the formation of a very potent inhibitor of lactate dehydrogenase. High resolution mass spectroscopy along with NMR characterization clearly showed that the inhibitor is derived from attachment of a glycolic acid moiety to the 4-position of the dihydronicotinamide ring of NADH. The very potent inhibitor is competitive with respect to NADH. The inhibitor added in submicromolar concentrations to cardiomyocytes protects them from damage caused by hypoxia/reoxygenation stress. In isolated mouse hearts, addition of the inhibitor results in a substantial reduction of myocardial infarct size caused by global ischemia/reperfusion injury.
NADH 的碱性孵育会形成一种非常有效的乳酸脱氢酶抑制剂。高分辨率质谱和 NMR 特征分析清楚地表明,抑制剂是由乙二醇酸部分附着在 NADH 的二氢烟酰胺环的 4 位上形成的。这种非常有效的抑制剂与 NADH 竞争。该抑制剂以亚毫摩尔浓度添加到心肌细胞中,可以保护它们免受缺氧/复氧应激引起的损伤。在分离的小鼠心脏中,加入该抑制剂可显著减少由整体缺血/再灌注损伤引起的心肌梗死面积。