Departamento de Ciencia e Tecnologia de Alimentos, Universidade Estadual de Londrina, CP 6001, 86051-970 Londrina-PR.
J Agric Food Chem. 2010 Jan 13;58(1):88-93. doi: 10.1021/jf9027427.
A reverse phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous quantification of kahweol and cafestol in tissues of fresh fruits, leaves, and roasted coffee beans. The best resolution was obtained with isocratic elution of acetonitrile/water (55/45% v/v) and UV detection. A single sample preparation method carried out by direct saponification and extraction with organic solvent was standardized for all matrices. Good recovery (average of 99% for kahweol and 94% for cafestol), repeatability, and linearity were obtained. Detection limits of 2.3 and 3.0 mg/100 g were observed for kahweol and cafestol. The HPLC method was effective in quantifying these diterpenes in the different coffee matrices. The endosperm and perisperm of Coffea arabica cv. IAPAR 59 showed elevated amounts of kahweol as compared to the pericarp and leaves. On the other hand, cafestol was detected in all samples except in leaves from Coffea canephora cv. Apoatā.
建立了反相高效液相色谱（HPLC）法，用于同时定量测定新鲜水果、叶片和烘焙咖啡豆组织中的卡瓦醇和咖啡醇。采用乙腈/水（55/45%v/v）等度洗脱和紫外检测，可获得最佳分辨率。针对所有基质，标准化了一种通过直接皂化和有机溶剂提取进行的单一样品制备方法。获得了良好的回收率（卡瓦醇的平均回收率为 99%，咖啡醇的平均回收率为 94%）、重现性和线性。卡瓦醇和咖啡醇的检测限分别为 2.3 和 3.0mg/100g。HPLC 法有效地定量分析了不同咖啡基质中的这些二萜。与咖啡果皮和叶片相比，阿拉比卡咖啡品种 IAPAR 59 的胚乳和胚乳含有较高量的卡瓦醇。另一方面，除了咖啡种 apoatā 的叶片外，所有样品中均检测到咖啡醇。
