分光荧光光度法测定商业剂型中盐酸多塞平的含量。
Spectrofluorimetric method for the determination of doxepin hydrochloride in commercial dosage forms.
机构信息
Department of Chemistry, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
出版信息
AAPS PharmSciTech. 2009;10(4):1381-7. doi: 10.1208/s12249-009-9341-z. Epub 2009 Nov 21.
A novel spectrofluorimetric method has been developed for the determination of doxepin hydrochloride in commercial dosage forms. The method is based on the fluorescent ion pair complex formation of the drug with eosin Y in the presence of sodium acetate-acetic acid buffer solution of pH 4.52 which is extractable in dichloromethane. The extracted complex showed fluorescence intensity at lambdaem=567 nm after excitation at 464 nm. The calibration curve was linear over the working range of 0.1-0.8 microg ml(-1). Under the optimized experimental conditions, present method is validated as per International Conference on Harmonization guidelines. The limit of detection for the developed method is 2.95 ng ml(-1). The method has been successfully applied to the determination of doxepin hydrochloride in commercial dosage forms. The results are compared with the reference spectrofluorimetric method.
一种新的分光荧光法已被开发用于测定盐酸多塞平在商业剂型中的含量。该方法基于药物与曙红 Y 在醋酸钠-乙酸缓冲溶液（pH 值为 4.52）中形成荧光离子对配合物，该配合物可在二氯甲烷中萃取。在 464nm 激发下，萃取的配合物在 lambdaem=567nm 处显示荧光强度。在 0.1-0.8μg ml(-1) 的工作范围内，校准曲线呈线性。在优化的实验条件下，本方法符合国际协调会议的指导原则进行了验证。该方法的检测限为 2.95ng ml(-1)。该方法已成功应用于商业剂型中盐酸多塞平的测定。结果与参考分光荧光法进行了比较。
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