聚合酶链反应中的扩展遗传字母。
Expanded genetic alphabets in the polymerase chain reaction.
机构信息
Foundation for Applied Molecular Evolution, 720 SW 2nd Avenue, Suite 201, Gainesville, FL 32601, USA.
出版信息
Angew Chem Int Ed Engl. 2010;49(1):177-80. doi: 10.1002/anie.200905173.
Abstract
Artificially expanded genetic information systems (AEGIS) add extra nucleotide "letters" to DNA alphabets; oligonucleotides containing AEGIS nucleotides do not bind to natural DNA. This "orthogonality" is exploited here by placing two AEGIS nucleotides (P and Z) in external tags for primers targeting three cancer genes in a nested PCR architecture. AEGIS tags support multiplexed PCR with fewer primer dimers and off-target amplicons than multiplexed PCR without AEGIS components.
摘要
人工扩展遗传信息系统（AEGIS）向 DNA 字母表中添加额外的核苷酸“字母”；含有 AEGIS 核苷酸的寡核苷酸与天然 DNA 不结合。在这里，通过在外引物的标签中放置两个 AEGIS 核苷酸（P 和 Z）来利用这种“正交性”，这些引物针对嵌套 PCR 结构中的三个癌症基因。与没有 AEGIS 成分的多重 PCR 相比，AEGIS 标签支持多重 PCR，其引物二聚体和非靶扩增子更少。
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