泰国人群中白细胞介素 12B 启动子等位基因 CTCTAA 与脑型疟疾易感性的关联复制研究。
A replication study of the association between the IL12B promoter allele CTCTAA and susceptibility to cerebral malaria in Thai population.
机构信息
Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
出版信息
Malar J. 2009 Dec 11;8:290. doi: 10.1186/1475-2875-8-290.
BACKGROUND
Interleukin-12 (IL-12), a heterodimeric cytokine composed of p35 and p40 subunits, has been thought to play an important role in the pathogenesis of malaria. The IL-12p40 subunit is encoded by the IL12B gene. An IL12B promoter allele, CTCTAA, at rs17860508 has been reported to be associated with susceptibility to cerebral malaria in African populations. However, this association has not so far been replicated in non-African populations.
METHODS
To examine whether the CTCTAA allele is associated with susceptibility to cerebral malaria in Asian populations, 303 Thai patients with Plasmodium falciparum malaria (109 cerebral malaria and 194 mild malaria patients) were genotyped for rs17860508 by PCR-direct sequencing.
RESULTS
The CTCTAA allele showed a significant association with susceptibility to cerebral malaria in the Thai population (allelic OR = 1.37; one sided P-value = 0.030).
CONCLUSIONS
The existence of a significant association between the CTCTAA allele and susceptibility to cerebral malaria was confirmed in Southeast Asian population, which was previously reported in African populations.
背景
白细胞介素-12（IL-12）是一种由 p35 和 p40 亚基组成的异二聚体细胞因子，被认为在疟疾的发病机制中发挥重要作用。IL-12p40 亚基由 IL12B 基因编码。据报道，IL12B 启动子等位基因 CTCTAA 位于 rs17860508 与非洲人群中脑型疟疾的易感性相关。然而，迄今为止，这种关联尚未在非非洲人群中得到复制。
方法
为了研究 CTCTAA 等位基因是否与亚洲人群中脑型疟疾的易感性相关，通过 PCR 直接测序对 303 例感染恶性疟原虫（109 例脑型疟疾和 194 例轻度疟疾患者）的泰国患者进行 rs17860508 基因分型。
结果
CTCTAA 等位基因与泰国人群中脑型疟疾的易感性显著相关（等位基因 OR = 1.37；单侧 P 值 = 0.030）。
结论
CTCTAA 等位基因与脑型疟疾易感性之间的显著关联在东南亚人群中得到了证实，这与之前在非洲人群中的报道一致。
Zotero 插件