洗必泰稳定黏接界面：一项为期两年的体外研究。
Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study.
机构信息
Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Via Stuparich 1, Trieste, Italy.
出版信息
Dent Mater. 2010 Apr;26(4):320-5. doi: 10.1016/j.dental.2009.11.153. Epub 2009 Dec 31.
OBJECTIVES
This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time.
METHODS
Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.2-2% CHX-treated dentin were obtained to assay dentin MMPs activity. Microtensile bond strength and interfacial nanoleakage expression of SB1XT bonded interfaces (with or without CHX pre-treatment for 30s on the etched surface) were analyzed immediately and after 2 years of storage in artificial saliva at 37 degrees C.
RESULTS
Zymograms showed that application of SB1XT to human dentin powder increases MMP-2 activity, while CHX pre-treatment inhibited all dentin gelatinolytic activity, irrespective from the tested concentration. CHX significantly lowered the loss of bond strength and nanoleakage seen in acid-etched resin-bonded dentin artificially aged for 2 years.
SIGNIFICANCE
The study demonstrates the active role of SB1XT in dentin MMP-2 activation and the efficacy of CHX inhibition of MMPs even if used at low concentration (0.2%).
目的
本研究评估了内源性牙本质基质金属蛋白酶（MMPs）在黏结界面胶原纤维自动降解中的作用。本研究的零假设是黏结剂混合物或洗必泰（CHX）的应用不会改变牙本质 MMPs 的活性，并且作为治疗性底漆的 CHX 不会随着时间的推移改善黏结界面的稳定性。
方法
从未经处理或 0.2%-2% CHX 处理过的牙本质粉末中提取蛋白提取物，通过蛋白电泳酶谱分析检测牙本质 MMPs 活性。对即刻和在 37°C 人工唾液中储存 2 年后的 SB1XT 黏结界面（在酸蚀表面上用或不用 CHX 预处理 30s）进行微拉伸黏结强度和界面纳米渗漏表达分析。
结果
电泳酶谱显示，将 SB1XT 应用于牙本质粉末可增加 MMP-2 活性，而 CHX 预处理抑制了所有牙本质明胶酶活性，与测试浓度无关。CHX 显著降低了经酸蚀处理的树脂黏结牙本质在人工老化 2 年后黏结强度丧失和纳米渗漏的增加。
结论
本研究表明 SB1XT 可激活牙本质 MMP-2，并且即使使用低浓度（0.2%）的 CHX 也能抑制 MMPs。
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