Boston, Mass.; and St. Louis, Mo. From the Department of Otology and Laryngology, Massachusetts Eye and Ear Infirmary, the Department of Surgery, Massachusetts General Hospital, Harvard Medical School, and the Division of Plastic and Reconstructive Surgery, Washington University School of Medicine.
Plast Reconstr Surg. 2010 Jan;125(1):99-109. doi: 10.1097/PRS.0b013e3181c2a5ea.
Measuring rodent facial movements is a reliable method for studying recovery from facial nerve manipulation and for examining the behavioral correlates of aberrant regeneration. The authors quantitatively compared recovery of vibrissal and ocular function following three types of clinically relevant nerve injury.
One hundred seventy-eight adult rats underwent facial nerve manipulation and testing. In the experimental groups, the left facial nerve was either crushed, transected, and repaired epineurially, or transected and the stumps suture-secured into a tube with a 2-mm gap between them. Facial recovery was measured for the ensuing 1 to 4 months. Data were analyzed for whisking recovery. Previously developed markers of co-contraction of the upper and midfacial zones (possible synkinesis markers) were also examined.
Animals in the crush groups recovered nearly normal whisking parameters within 25 days. The distal branch crush group showed improved recovery over the main trunk crush group for several days during early recovery. By week 9, the transection/repair groups showed evidence of recovery that trended further upward throughout the study period. The entubulation groups followed a similar recovery pattern, although they did not maintain significant recovery levels by the study conclusion. Markers of potential synkinesis increased in selected groups following facial nerve injury.
Rodent vibrissal function recovers in a predictable fashion following manipulation. Generalized co-contraction of the upper and midfacial zones emerges following facial nerve manipulation, possibly related to aberrant regeneration, polyterminal axons, or hypersensitivity of the rodent to sensory stimuli following nerve manipulation.
测量啮齿动物的面部运动是研究面神经操作后恢复情况以及研究异常再生的行为相关性的可靠方法。作者定量比较了三种临床相关神经损伤后触须和眼部功能的恢复情况。
178 只成年大鼠接受面神经操作和测试。在实验组中，左侧面神经或被挤压、横断并用外膜修复，或横断并用 2mm 间隙的管将神经残端缝合固定。接下来的 1 到 4 个月对面神经恢复情况进行测量。分析数据以评估触须恢复情况。还检查了先前开发的用于评估上下面部区域共同收缩（可能的联带运动标志物）的标记物。
挤压组的动物在 25 天内几乎恢复了正常的触须参数。在早期恢复期间，远端分支挤压组的恢复情况优于主干挤压组。到第 9 周，横断/修复组表现出的恢复趋势在整个研究期间进一步上升。套管组也遵循类似的恢复模式，尽管在研究结束时它们没有保持显著的恢复水平。面神经损伤后，一些选定组的潜在联带运动标志物增加。
啮齿动物触须功能在操作后以可预测的方式恢复。面神经操作后出现上、中面部区域的普遍共同收缩，可能与异常再生、多末端轴突或神经操作后啮齿动物对感觉刺激的过度敏感有关。
