分析脐血源 CD34 细胞体外扩增和中性粒细胞分化过程中 Wnt 通路基因的表达。
Analysis of Wnt pathway genes during ex vivo expansion and neutrophil differentiation of umbilical-cord-blood-derived CD34 cells.
机构信息
Scottish National Blood Transfusion Service, Cell Therapy Group, MRC Centre for Regenerative Medicine, 49 Little France Crescent, Edinburgh, UK.
出版信息
Vox Sang. 2010 Apr;98(3 Pt 1):e290-4. doi: 10.1111/j.1423-0410.2009.01299.x. Epub 2010 Jan 12.
Previous work has shown that optimal ex vivo expansion and differentiation of CD34(+) progenitor cells into neutrophils is by addition of Flt3-L, SCF and G-CSF. Here we report that a variety of genes involved in the WNT pathway are transcriptionally active in both undifferentiated and differentiated umbilical cord blood CD34(+) cells, however statistically significant changes in gene expression are not always consistent across UCB samples.
先前的研究表明，通过添加 Flt3-L、SCF 和 G-CSF，可实现 CD34(+)祖细胞的最佳离体扩增和向中性粒细胞的分化。在此，我们报告称，WNT 通路中多种基因在未分化和分化的脐带血 CD34(+)细胞中均具有转录活性，但 UCB 样本中的基因表达变化并不总是一致的，统计学上无显著差异。
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