宫颈癌患者中用于检测人乳头瘤病毒的配对宫颈刮片与肿瘤组织样本的比较。
Comparison of paired cervical scrape and tumor tissue samples for detection of human papillomaviruses in patients with cervical cancer.
作者信息
Jancar N, Kocjan B J, Poljak M, Bokal E Vrtacnik
机构信息
Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
出版信息
Eur J Gynaecol Oncol. 2009;30(6):675-8.
PURPOSE OF INVESTIGATION
To compare the detection and distribution of HPV genotypes in paired cervical scrape samples and tumor tissue samples in patients with cervical cancer.
METHODS
Forty cervical scrape samples and 40 paired archival or fresh frozen tissue samples were collected from women with cervical cancer. Polymerase chain reaction with GP5+ and GP6+ primers was performed in all samples for HPV DNA detection. All GP5+/GP6+ negative samples were additionally tested using INNO-LiPA HPV Genotyping Extra Test.
RESULTS
Overall, 39/40 (97.5%) of CC samples were HPV DNA positive. HPV 16 was found in 24/40 samples, HPV 18 in 5/40 samples. A co-infection with two different HPV genotypes was identified in one cervical scrape specimen, while in tissue samples only single infections were detected. Overall agreement between paired samples was 98.75%.
CONCLUSION
The present study has shown that cervical scrape samples are equally useful for HPV genotype determination as tumor tissue samples in patients with cervical cancer. They can be used as accurate clinical samples for detection of HPV genotype causing cervical cancer or for epidemiological molecular studies.
研究目的
比较宫颈癌患者配对的宫颈刮片样本和肿瘤组织样本中HPV基因型的检测及分布情况。
方法
从宫颈癌女性患者中收集40份宫颈刮片样本以及40份配对的存档或新鲜冷冻组织样本。对所有样本进行聚合酶链反应，使用GP5 +和GP6 +引物检测HPV DNA。所有GP5 + / GP6 +阴性样本另外使用INNO - LiPA HPV基因分型额外检测法进行检测。
结果
总体而言，40份宫颈癌（CC）样本中有39份（97.5%）HPV DNA呈阳性。40份样本中，24份检测出HPV 16，5份检测出HPV 18。在一份宫颈刮片标本中鉴定出两种不同HPV基因型的合并感染，而在组织样本中仅检测到单一感染。配对样本之间的总体一致性为98.75%。
结论
本研究表明，对于宫颈癌患者，宫颈刮片样本在确定HPV基因型方面与肿瘤组织样本同样有用。它们可作为准确的临床样本，用于检测导致宫颈癌的HPV基因型或进行流行病学分子研究。
Zotero 插件