通过阻断蝾螈视网膜中D-丝氨酸的合成，光诱发的NMDA受体介导的电流会减少。
Light-evoked NMDA receptor-mediated currents are reduced by blocking D-serine synthesis in the salamander retina.
作者信息
Stevens Eric R, Gustafson Eric C, Sullivan Steven J, Esguerra Manuel, Miller Robert F
机构信息
Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA.
出版信息
Neuroreport. 2010 Mar 10;21(4):239-44. doi: 10.1097/WNR.0b013e32833313b7.
Abstract
Experiments were carried out in the retina of the tiger salamander (Ambystoma tigrinum) to evaluate the importance of D-serine synthesis on light-evoked N-methyl D-aspartate (NMDA) receptor-mediated components of ganglion cells and contributions to the proximal negative field potential. We blocked the synthesis of D-serine through brief exposures of the retina to phenazine ethosulfate and validated the changes in the tissue levels of D-serine using capillary electrophoresis methods to separate and measure the amino acid enantiomers. Ten minute exposures to phenazine ethosulfate decreased D-serine levels in the retina by about 50% and significantly reduced the NMDA receptor contribution to light responses of the inner retina. This is the first report of a linkage between D-serine synthesis and NMDA receptor activity in the vertebrate retina.
摘要
在虎蝾螈（Ambystoma tigrinum）的视网膜上进行了实验，以评估D-丝氨酸合成对光诱发的神经节细胞N-甲基-D-天冬氨酸（NMDA）受体介导成分的重要性，以及对近端负场电位的贡献。我们通过将视网膜短暂暴露于吩嗪硫酸乙酯来阻断D-丝氨酸的合成，并使用毛细管电泳方法分离和测量氨基酸对映体，以验证D-丝氨酸组织水平的变化。暴露于吩嗪硫酸乙酯10分钟可使视网膜中的D-丝氨酸水平降低约50%，并显著降低NMDA受体对视网膜内层光反应的贡献。这是脊椎动物视网膜中D-丝氨酸合成与NMDA受体活性之间联系的首次报道。
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