Larsen Helga S, Ruus Ann-Kristin, Schreurs Olav, Galtung Hilde Kanli
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
Eur J Oral Sci. 2010 Feb;118(1):9-13. doi: 10.1111/j.1600-0722.2009.00708.x.
Several aquaporins (AQPs) have been detected in mature and embryonic mammalian salivary glands (AQP1 and AQP3-AQP8). However, AQP11 has, to our knowledge, never before been described in salivary glands, but is known to be important in, for example, kidney development in mice. We therefore thought it relevant to investigate if AQP11 was present during salivary organogenesis. The submandibular salivary gland (SMG) from CD1 mice was studied during prenatal development and early postnatal development, and also in young adult male and female mice. The expression trend of the AQP11 transcript was detected using the reverse transcription-polymerase chain reaction (RT-PCR), and the temporal-spatial pattern was observed using in situ hybridization. The AQP11 transcript was first detected at embryonic day 13.5 and showed a more or less constitutive expression trend during the prenatal and early postnatal SMG development. Spatial studies demonstrated that the AQP11 transcript was present in the developing and mature duct structures at all stages studied. In the end pieces, the AQP11 transcript was reduced during glandular development. Our results point to an important role for AQP11 during salivary gland development.
在成熟和胚胎期的哺乳动物唾液腺中已检测到几种水通道蛋白（AQP）（AQP1和AQP3 - AQP8）。然而，据我们所知，AQP11此前从未在唾液腺中被描述过，但已知其在例如小鼠肾脏发育中很重要。因此，我们认为研究AQP11在唾液腺器官发生过程中是否存在具有相关性。对CD1小鼠的下颌下唾液腺（SMG）在产前发育、出生后早期发育阶段以及成年雌雄小鼠中进行了研究。使用逆转录 - 聚合酶链反应（RT - PCR）检测AQP11转录本的表达趋势，并通过原位杂交观察其时空模式。AQP11转录本在胚胎第13.5天首次被检测到，并且在产前和出生后早期SMG发育过程中呈现出或多或少的组成型表达趋势。空间研究表明，在所研究的所有阶段，AQP11转录本都存在于发育中的和成熟的导管结构中。在终末段，AQP11转录本在腺体发育过程中减少。我们的结果表明AQP11在唾液腺发育过程中起重要作用。
