BACH1/FANCJ 与 TopBP1 相互作用，并参与早期的 DNA 复制检验点控制。
BACH1/FANCJ acts with TopBP1 and participates early in DNA replication checkpoint control.
机构信息
Department of Experimental Radiation Oncology, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
出版信息
Mol Cell. 2010 Feb 12;37(3):438-46. doi: 10.1016/j.molcel.2010.01.002.
Abstract
Human TopBP1 plays a critical role in the control of DNA replication checkpoint. In this study, we report a specific interaction between TopBP1 and BACH1/FANCJ, a DNA helicase involved in the repair of DNA crosslinks. The TopBP1/BACH1 interaction is mediated by the very C-terminal tandem BRCT domains of TopBP1 and S phase-specific phosphorylation of BACH1 at Thr 1133 site. Interestingly, we demonstrate that depletion of TopBP1 or BACH1 attenuates the loading of RPA on chromatin. Moreover, both TopBP1 and BACH1 are required for ATR-dependent phosphorylation events in response to replication stress. Taken together, our data suggest that BACH1 has an unexpected early role in replication checkpoint control. A specific interaction between TopBP1 and BACH1 is likely to be required for the extension of single-stranded DNA regions and RPA loading following replication stress, which is a prerequisite for the subsequent activation of replication checkpoint.
摘要
人类 TopBP1 在控制 DNA 复制检查点方面起着关键作用。在这项研究中，我们报告了 TopBP1 与 BACH1/FANCJ 之间的特定相互作用，BACH1/FANCJ 是一种参与 DNA 交联修复的 DNA 解旋酶。TopBP1/BACH1 相互作用是由 TopBP1 的非常 C 末端串联 BRCT 结构域和 S 期特有的 BACH1 第 Thr1133 位丝氨酸磷酸化介导的。有趣的是，我们证明了 TopBP1 或 BACH1 的耗竭会减弱 RPA 在染色质上的加载。此外，TopBP1 和 BACH1 都需要 ATR 依赖性磷酸化事件来响应复制应激。总之，我们的数据表明，BACH1 在复制检查点控制中具有意想不到的早期作用。TopBP1 和 BACH1 之间的特定相互作用可能是复制应激后单链 DNA 区域延伸和 RPA 加载所必需的，这是随后复制检查点激活的前提。
相似文献
1
BACH1/FANCJ acts with TopBP1 and participates early in DNA replication checkpoint control.BACH1/FANCJ 与 TopBP1 相互作用，并参与早期的 DNA 复制检验点控制。
Mol Cell. 2010 Feb 12;37(3):438-46. doi: 10.1016/j.molcel.2010.01.002.
2
CtIP- and ATR-dependent FANCJ phosphorylation in response to DNA strand breaks mediated by DNA replication.CtIP- 和 ATR 依赖性 FANCJ 磷酸化响应由 DNA 复制介导的 DNA 链断裂。
Genes Cells. 2012 Dec;17(12):962-70. doi: 10.1111/gtc.12011. Epub 2012 Nov 15.
3
Molecular basis of BACH1/FANCJ recognition by TopBP1 in DNA replication checkpoint control.BACH1/FANCJ 被 TopBP1 识别的分子基础在 DNA 复制检验点控制中。
J Biol Chem. 2011 Feb 11;286(6):4292-301. doi: 10.1074/jbc.M110.189555. Epub 2010 Dec 2.
4
Spatial organization and functions of Chk1 activation by TopBP1 biomolecular condensates.TopBP1 生物分子凝聚物对 Chk1 激活的空间组织和功能。
Cell Rep. 2024 Apr 23;43(4):114064. doi: 10.1016/j.celrep.2024.114064. Epub 2024 Apr 4.
5
FANCJ (BACH1) helicase forms DNA damage inducible foci with replication protein A and interacts physically and functionally with the single-stranded DNA-binding protein.FANCJ（BACH1）解旋酶与复制蛋白A形成DNA损伤诱导灶，并与单链DNA结合蛋白在物理和功能上相互作用。
Blood. 2007 Oct 1;110(7):2390-8. doi: 10.1182/blood-2006-11-057273. Epub 2007 Jun 27.
6
Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling.RPA 覆盖的单链 DNA 激活的 ATR-Chk1 信号的重建。
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13660-5. doi: 10.1073/pnas.1007856107. Epub 2010 Jun 28.
7
A DNA damage-regulated BRCT-containing protein, TopBP1, is required for cell survival.一种受DNA损伤调节的含BRCT结构域蛋白TopBP1是细胞存活所必需的。
Mol Cell Biol. 2002 Jan;22(2):555-66. doi: 10.1128/MCB.22.2.555-566.2002.
8
ATR autophosphorylation as a molecular switch for checkpoint activation.ATR 自身磷酸化作为检查点激活的分子开关。
Mol Cell. 2011 Jul 22;43(2):192-202. doi: 10.1016/j.molcel.2011.06.019.
9
Direct role for the replication protein treslin (Ticrr) in the ATR kinase-mediated checkpoint response.复制蛋白 treslin（Ticrr）在 ATR 激酶介导的检查点反应中的直接作用。
J Biol Chem. 2013 Jun 28;288(26):18903-10. doi: 10.1074/jbc.M113.475517. Epub 2013 May 21.
10
Interaction between Rad9-Hus1-Rad1 and TopBP1 activates ATR-ATRIP and promotes TopBP1 recruitment to sites of UV-damage.Rad9-Hus1-Rad1与TopBP1之间的相互作用激活了ATR-ATRIP，并促进TopBP1募集到紫外线损伤位点。
DNA Repair (Amst). 2014 Sep;21:1-11. doi: 10.1016/j.dnarep.2014.05.001. Epub 2014 May 27.
引用本文的文献
1
Thirty Years of BRCA1: Mechanistic Insights and Their Impact on Mutation Carriers.BRCA1的三十年：机制洞察及其对突变携带者的影响
Cancer Discov. 2025 Mar 3;15(3):461-480. doi: 10.1158/2159-8290.CD-24-1326.
2
BRCA1 and BRCA2: from cancer susceptibility to synthetic lethality.BRCA1和BRCA2：从癌症易感性到合成致死性
Genes Dev. 2025 Jan 7;39(1-2):86-108. doi: 10.1101/gad.352083.124.
3
A TRilogy of ATR's Non-Canonical Roles Throughout the Cell Cycle and Its Relation to Cancer.ATR在整个细胞周期中的非经典作用三部曲及其与癌症的关系
Cancers (Basel). 2024 Oct 19;16(20):3536. doi: 10.3390/cancers16203536.
4
Systematic investigation of BRCA1-A, -B, and -C complexes and their functions in DNA damage response and DNA repair.系统研究 BRCA1-A、-B 和 -C 复合物及其在 DNA 损伤反应和 DNA 修复中的功能。
Oncogene. 2024 Aug;43(35):2621-2634. doi: 10.1038/s41388-024-03108-y. Epub 2024 Jul 27.
5
Purification and biochemical characterization of the G4 resolvase and DNA helicase FANCJ.G4 解旋酶和 FANCJ DNA 解旋酶的纯化及生化特性分析
Methods Enzymol. 2024;695:1-27. doi: 10.1016/bs.mie.2023.12.006. Epub 2024 Jan 5.
6
The DNA helicase FANCJ (BRIP1) functions in double strand break repair processing, but not crossover formation during prophase I of meiosis in male mice.DNA 解旋酶 FANCJ（BRIP1）在雄性小鼠减数分裂前期的双链断裂修复处理中发挥作用，但不参与交叉形成。
PLoS Genet. 2024 Feb 20;20(2):e1011175. doi: 10.1371/journal.pgen.1011175. eCollection 2024 Feb.
7
FANCJ DNA helicase is recruited to the replisome by AND-1 to ensure genome stability.FANCJ 解旋酶通过 AND-1 招募到复制体，以确保基因组的稳定性。
EMBO Rep. 2024 Feb;25(2):876-901. doi: 10.1038/s44319-023-00044-y. Epub 2024 Jan 2.
8
The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice.DNA解旋酶FANCJ（BRIP1）在双链断裂修复过程中发挥作用，但在雄性小鼠减数分裂前期I的交叉形成过程中不起作用。
bioRxiv. 2023 Oct 8:2023.10.06.561296. doi: 10.1101/2023.10.06.561296.
9
ADAR1 links R-loop homeostasis to ATR activation in replication stress response.ADAR1 将 R 环平衡与复制应激反应中的 ATR 激活联系起来。
Nucleic Acids Res. 2023 Nov 27;51(21):11668-11687. doi: 10.1093/nar/gkad839.
10
Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens.通过表型 CRISPR-Cas9 筛选对 DNA 损伤抑制剂进行全基因组图谱绘制。
Mol Cell. 2023 Aug 3;83(15):2792-2809.e9. doi: 10.1016/j.molcel.2023.06.025. Epub 2023 Jul 20.
本文引用的文献
1
TopBP1 and DNA polymerase-alpha directly recruit the 9-1-1 complex to stalled DNA replication forks.TopBP1和DNA聚合酶α直接将9-1-1复合物招募至停滞的DNA复制叉处。
J Cell Biol. 2009 Mar 23;184(6):793-804. doi: 10.1083/jcb.200810185. Epub 2009 Mar 16.
2
FANCJ is a structure-specific DNA helicase associated with the maintenance of genomic G/C tracts.FANCJ是一种与基因组G/C区域维持相关的结构特异性DNA解旋酶。
J Biol Chem. 2008 Dec 26;283(52):36132-9. doi: 10.1074/jbc.M808152200. Epub 2008 Oct 31.
3
ATR: an essential regulator of genome integrity.ATR：基因组完整性的关键调节因子。
Nat Rev Mol Cell Biol. 2008 Aug;9(8):616-27. doi: 10.1038/nrm2450. Epub 2008 Jul 2.
4
TopBP1 activates ATR through ATRIP and a PIKK regulatory domain.TopBP1通过ATRIP和一个PIKK调节结构域激活ATR。
Genes Dev. 2008 Jun 1;22(11):1478-89. doi: 10.1101/gad.1666208.
5
How ATR turns on: TopBP1 goes on ATRIP with ATR.ATR如何激活：TopBP1与ATR结合并作用于ATRIP。
Genes Dev. 2008 Jun 1;22(11):1416-21. doi: 10.1101/gad.1685108.
6
FANCJ helicase defective in Fanconia anemia and breast cancer unwinds G-quadruplex DNA to defend genomic stability.在范可尼贫血和乳腺癌中存在缺陷的FANCJ解旋酶可解开G-四链体DNA以维护基因组稳定性。
Mol Cell Biol. 2008 Jun;28(12):4116-28. doi: 10.1128/MCB.02210-07. Epub 2008 Apr 21.
7
Mechanisms to control rereplication and implications for cancer.控制再复制的机制及其对癌症的影响。
Curr Opin Cell Biol. 2007 Dec;19(6):663-71. doi: 10.1016/j.ceb.2007.10.007. Epub 2007 Nov 28.
8
Emergence of a DNA-damage response network consisting of Fanconi anaemia and BRCA proteins.由范可尼贫血蛋白和乳腺癌易感基因（BRCA）蛋白组成的DNA损伤反应网络的出现。
Nat Rev Genet. 2007 Oct;8(10):735-48. doi: 10.1038/nrg2159. Epub 2007 Sep 4.
9
Activation of BRCA1/BRCA2-associated helicase BACH1 is required for timely progression through S phase.通过S期及时进展需要激活BRCA1/BRCA2相关解旋酶BACH1。
Mol Cell Biol. 2007 Oct;27(19):6733-41. doi: 10.1128/MCB.00961-07. Epub 2007 Jul 30.
10
The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR.Rad9-Hus1-Rad1检查点钳调控TopBP1与ATR的相互作用。
J Biol Chem. 2007 Sep 21;282(38):28036-44. doi: 10.1074/jbc.M704635200. Epub 2007 Jul 18.
Zotero 插件