随机扩增多态 DNA-序列特征扩增区域标记的开发，用于鉴定夹竹桃科白麻属白麻和罗布麻属罗布麻。

Development of randomly amplified polymorphic DNA-sequence characterized amplified region marker for identification of Apocynum venetum LINN. from A. pictum SCHRENK.

机构信息

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, China.

出版信息

Biol Pharm Bull. 2010;33(3):522-6. doi: 10.1248/bpb.33.522.

DOI:10.1248/bpb.33.522

PMID:20190420

Abstract

Apocynum venetum LINN. is an important Chinese crude drug, and its sibling species A. pictum SCHRENK is a confusable herb which is similar to it. The purpose of this study is to develop DNA molecular markers to distinguish A. venetum from A. pictum through the combinative technologies of bulked segregate analysis (BSA) and randomly amplified polymorphic DNA (RAPD). Two putative markers B08-407 and B03-1368 specific for A. venetum were identified and sequenced. Based on the sequence information, two pairs of primers were designed and synthesized for sequence characterized amplified region (SCAR) markers. But only one primer pair, B03-1368, produced a clear SCAR band in all samples of A. venetum and not in A. pictum. This SCAR marker was found useful for rapid identification of A. venetum from A. pictum.

摘要

宿根野麻是一种重要的中国中药材，其近缘种罗布麻也是一种容易混淆的相似草药。本研究旨在通过结合分离群体分析（BSA）和随机扩增多态性 DNA（RAPD）技术，开发 DNA 分子标记，以区分宿根野麻和罗布麻。本研究通过分离群体分析和随机扩增多态性 DNA 技术，从宿根野麻和罗布麻的混合 DNA 中筛选出与宿根野麻特异连锁的分子标记，得到了 2 个与宿根野麻连锁的 RAPD 标记 B08-407 和 B03-1368，并对其进行了测序和序列分析。根据测序结果，设计并合成了针对这 2 个标记的 SCAR 引物，只有引物 B03-1368 在所有供试的宿根野麻样本中扩增出了清晰的 SCAR 条带，而在罗布麻样本中没有扩增出条带。该 SCAR 标记可用于快速鉴定宿根野麻和罗布麻。