蒺藜果实成分可预防他克林诱导的 HepG2 细胞毒性。

Tribuli fructus constituents protect against tacrine-induced cytotoxicity in HepG2 cells.

机构信息

Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 570-749, Korea.

出版信息

Arch Pharm Res. 2010 Jan;33(1):67-70. doi: 10.1007/s12272-010-2226-6. Epub 2010 Feb 27.

PMID:20191345

Abstract

A new phenolic amide, tribulusimide D (4-hydroxy-N-[3-(4-hydroxy-3-methoxyphenyl)-1-oxo-2-propen-1-yl]-3-methoxybenzamide) (1), together with a known phenolic amide, terrestriamide ((E)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-2-oxoethyl]-prop-2-enamide) (2) and a flavonol glycoside, quercetin-3-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (3) were isolated from the H2O extract of Tribuli Fructus. Compounds 1 and 3 showed significant hepatoprotective activities, with EC50 values of 13.46 +/- 0.2 and 7.06 +/- 0.7 microM, respectively, against tacrine-induced cytotoxicity in HepG2 cells.

摘要

从蒺藜果实的 H2O 提取物中分离得到了一种新的酚酰胺化合物，即三裂叶薯酰胺 D（4-羟基-N-[3-（4-羟基-3-甲氧基苯基）-1-氧代-2-丙烯基]-3-甲氧基苯甲酰胺）（1），以及一种已知的酚酰胺化合物，地胆酰胺（（E）-3-（4-羟基-3-甲氧基苯基）-N-[2-（4-羟基苯基）-2-氧代乙基]-丙烯酰胺）（2）和一种黄酮醇糖苷，槲皮素-3-O-β-D-吡喃葡萄糖基-（1'->6）-β-D-吡喃葡萄糖苷（3）。化合物 1 和 3 对 Tacrine 诱导的 HepG2 细胞毒性表现出显著的肝保护活性，EC50 值分别为 13.46±0.2 和 7.06±0.7 μM。