使用高分辨率熔解分析技术在 Rotor-Gene 6000 仪器上对烟曲霉 Cyp51A 基因的 54 号密码子进行特征分析。
Use of a high-resolution melt assay to characterize codon 54 of the cyp51A gene of Aspergillus fumigatus on a Rotor-Gene 6000 instrument.
机构信息
Cleveland Clinic, Cleveland, OH 44195, USA.
出版信息
Antimicrob Agents Chemother. 2010 May;54(5):2248-51. doi: 10.1128/AAC.01712-09. Epub 2010 Mar 1.
Abstract
A high-resolution melt (HRM) assay using a Rotor-Gene 6000 instrument was developed to characterize the codon for glycine 54 in the cyp51A genes from 13 reference isolates and 12 clinical isolates of Aspergillus fumigatus. Mutations in this codon confer reduced susceptibility to itraconazole and posaconazole. The assay is simple to perform, and a result of "wild type" or "mutant" is available after approximately 1 h following DNA extraction using commercially available reagents and conventional primers.
摘要
建立了一种基于 Rotor-Gene 6000 仪器的高分辨率熔解（HRM）分析方法，用于分析 13 个参考分离株和 12 个临床分离株的烟曲霉 Cyp51A 基因中甘氨酸 54 密码子的特征。该密码子的突变导致对伊曲康唑和泊沙康唑的敏感性降低。该分析方法操作简单，使用市售试剂和常规引物提取 DNA 后，大约 1 小时即可得到“野生型”或“突变型”的结果。
相似文献
1
Use of a high-resolution melt assay to characterize codon 54 of the cyp51A gene of Aspergillus fumigatus on a Rotor-Gene 6000 instrument.使用高分辨率熔解分析技术在 Rotor-Gene 6000 仪器上对烟曲霉 Cyp51A 基因的 54 号密码子进行特征分析。
Antimicrob Agents Chemother. 2010 May;54(5):2248-51. doi: 10.1128/AAC.01712-09. Epub 2010 Mar 1.
2
Detection of Aspergillus fumigatus and a mutation that confers reduced susceptibility to itraconazole and posaconazole by real-time PCR and pyrosequencing.通过实时聚合酶链反应和焦磷酸测序法检测烟曲霉及其对伊曲康唑和泊沙康唑敏感性降低的突变。
J Clin Microbiol. 2005 Feb;43(2):906-8. doi: 10.1128/JCM.43.2.906-908.2005.
3
Aspergillus fumigatus strains with mutations in the cyp51A gene do not always show phenotypic resistance to itraconazole, voriconazole, or posaconazole.烟曲霉中细胞色素 P45051A 基因突变株并不总是表现出对伊曲康唑、伏立康唑或泊沙康唑的表型耐药性。
Antimicrob Agents Chemother. 2011 May;55(5):2460-2. doi: 10.1128/AAC.01358-10. Epub 2011 Feb 14.
4
Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazole.用于鉴定烟曲霉cyp51A基因中赋予对伊曲康唑耐药性的突变的快速、高通量、多重、实时PCR。
J Clin Microbiol. 2005 Jan;43(1):214-22. doi: 10.1128/JCM.43.1.214-222.2005.
5
Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods.通过商业梯度试验（Etest）和参考方法测定烟曲霉分离株的cyp51A序列多态性与对三唑类药物敏感性之间的关系。
Acta Biochim Pol. 2017;64(4):631-634. doi: 10.18388/abp.2017_1571. Epub 2017 Nov 30.
6
In vitro susceptibility of Aspergillus fumigatus to isavuconazole: correlation with itraconazole, voriconazole, and posaconazole.烟曲霉对伊曲康唑、伏立康唑和泊沙康唑的体外药敏性：与伏立康唑的相关性。
Antimicrob Agents Chemother. 2013 Nov;57(11):5778-80. doi: 10.1128/AAC.01141-13. Epub 2013 Sep 16.
7
Quantitative Analysis of Single-Nucleotide Polymorphism for Rapid Detection of TR34/L98H- and TR46/Y121F/T289A-Positive Aspergillus fumigatus Isolates Obtained from Patients in Iran from 2010 to 2014.2010年至2014年从伊朗患者中分离出的烟曲霉TR34/L98H和TR46/Y121F/T289A阳性菌株的单核苷酸多态性定量分析用于快速检测
Antimicrob Agents Chemother. 2015 Nov 2;60(1):387-92. doi: 10.1128/AAC.02326-15. Print 2016 Jan.
8
Azole resistance in Aspergillus fumigatus isolates from the ARTEMIS global surveillance study is primarily due to the TR/L98H mutation in the cyp51A gene.烟曲霉分离株中唑类耐药性主要归因于 CYP51A 基因中的 TR/L98H 突变，这是 ARTEMIS 全球监测研究的结果。
Antimicrob Agents Chemother. 2011 Sep;55(9):4465-8. doi: 10.1128/AAC.00185-11. Epub 2011 Jun 20.
9
First determination of azole resistance in Aspergillus fumigatus strains carrying the TR34/L98H mutations in Turkey.在土耳其首次测定携带TR34/L98H突变的烟曲霉菌株对唑类药物的耐药性。
J Infect Chemother. 2015 Aug;21(8):581-6. doi: 10.1016/j.jiac.2015.04.012. Epub 2015 May 14.
10
Mutations in the cyp51A gene and susceptibility to itraconazole in Aspergillus fumigatus serially isolated from a patient with lung aspergilloma.从一名肺曲菌球患者连续分离出的烟曲霉中cyp51A基因的突变及其对伊曲康唑的敏感性
J Antimicrob Chemother. 2005 Jan;55(1):31-7. doi: 10.1093/jac/dkh507. Epub 2004 Nov 24.
引用本文的文献
1
An update on current and novel molecular diagnostics for the diagnosis of invasive fungal infections.侵袭性真菌感染诊断中当前及新型分子诊断方法的最新进展。
Expert Rev Mol Diagn. 2023 Jul-Dec;23(12):1135-1152. doi: 10.1080/14737159.2023.2267977. Epub 2023 Dec 15.
2
Detecting Azole-Antifungal Resistance in by Pyrosequencing.通过焦磷酸测序法检测念珠菌中的唑类抗真菌药物耐药性 。
需注意，你提供的原文“Detecting Azole-Antifungal Resistance in by Pyrosequencing.”似乎不完整，“in”后面缺少具体内容，我是按照合理推测补充完整后翻译的。
J Fungi (Basel). 2020 Jan 10;6(1):12. doi: 10.3390/jof6010012.
3
Development and Validation of a High-Resolution Melting Assay To Detect Azole Resistance in Aspergillus fumigatus.开发和验证一种用于检测烟曲霉唑类耐药性的高分辨率熔解分析方法。
Antimicrob Agents Chemother. 2017 Nov 22;61(12). doi: 10.1128/AAC.01083-17. Print 2017 Dec.
4
Detection of B Gene Mutations in SDHI-Resistant Isolates of Using High Resolution Melting (HRM) Analysis.利用高分辨率熔解曲线分析（HRM）检测对琥珀酸脱氢酶抑制剂（SDHI）耐药菌株中的B基因突变
Front Microbiol. 2016 Nov 15;7:1815. doi: 10.3389/fmicb.2016.01815. eCollection 2016.
5
Rapid detection and identification of Aspergillus from lower respiratory tract specimens by use of a combined probe-high-resolution melting analysis.采用组合探针-高分辨率熔解分析技术快速检测和鉴定下呼吸道标本中的曲霉属。
J Clin Microbiol. 2012 Oct;50(10):3238-43. doi: 10.1128/JCM.00176-12. Epub 2012 Jul 25.
6
Antifungal drug resistance: do molecular methods provide a way forward?抗真菌药物耐药性：分子方法是否提供了前进的道路？
Curr Opin Infect Dis. 2009 Dec;22(6):568-73. doi: 10.1097/QCO.0b013e3283321ce5.
本文引用的文献
1
Frequency and evolution of Azole resistance in Aspergillus fumigatus associated with treatment failure.烟曲霉中唑类耐药与治疗失败相关的频率及演变
Emerg Infect Dis. 2009 Jul;15(7):1068-76. doi: 10.3201/eid1507.090043.
2
Emergence of azole resistance in Aspergillus fumigatus and spread of a single resistance mechanism.烟曲霉中唑类耐药性的出现及单一耐药机制的传播。
PLoS Med. 2008 Nov 11;5(11):e219. doi: 10.1371/journal.pmed.0050219.
3
In vitro survey of triazole cross-resistance among more than 700 clinical isolates of Aspergillus species.700多株曲霉菌临床分离株的三唑类交叉耐药性体外研究。
J Clin Microbiol. 2008 Aug;46(8):2568-72. doi: 10.1128/JCM.00535-08. Epub 2008 Jun 18.
4
High resolution melting applications for clinical laboratory medicine.临床检验医学中的高分辨率熔解应用
Exp Mol Pathol. 2008 Aug;85(1):50-8. doi: 10.1016/j.yexmp.2008.03.012. Epub 2008 Apr 13.
5
Fluorometric assay for testing rifampin susceptibility of Mycobacterium tuberculosis complex.用于检测结核分枝杆菌复合群对利福平敏感性的荧光测定法。
J Clin Microbiol. 2008 Apr;46(4):1369-73. doi: 10.1128/JCM.02343-07. Epub 2008 Feb 27.
6
Rapid detection of triazole antifungal resistance in Aspergillus fumigatus.烟曲霉中三唑类抗真菌药物耐药性的快速检测
J Clin Microbiol. 2008 Apr;46(4):1200-6. doi: 10.1128/JCM.02330-07. Epub 2008 Jan 30.
7
High-resolution melting analysis of the spa repeat region of Staphylococcus aureus.金黄色葡萄球菌spa重复区域的高分辨率熔解分析。
Clin Chem. 2008 Feb;54(2):432-6. doi: 10.1373/clinchem.2007.093658.
8
Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis.通过高分辨率熔解分析快速区分甲型流感病毒亚型并对病毒变体进行基因筛查。
J Clin Microbiol. 2008 Mar;46(3):1090-7. doi: 10.1128/JCM.02015-07. Epub 2008 Jan 3.
9
Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region.利用单链构象多态性和vlhA基因单拷贝区域的高分辨率熔解曲线分析对滑膜支原体菌株进行分类。
Microbiology (Reading). 2007 Aug;153(Pt 8):2679-2688. doi: 10.1099/mic.0.2006/005140-0.
10
Optimization of high-resolution melting analysis for low-cost and rapid screening of allelic variants of Bacillus anthracis by multiple-locus variable-number tandem repeat analysis.通过多位点可变数目串联重复序列分析对炭疽芽孢杆菌等位基因变体进行低成本快速筛选的高分辨率熔解分析优化。
Clin Chem. 2007 Jul;53(7):1377-80. doi: 10.1373/clinchem.2007.085993. Epub 2007 May 24.
Zotero 插件