经皮接种微针涂层丙型肝炎 DNA 疫苗。
Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine.
机构信息
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
出版信息
Gene Ther. 2010 Jun;17(6):811-4. doi: 10.1038/gt.2010.22. Epub 2010 Mar 4.
Abstract
The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.
摘要
皮肤作为一种可及的组织，其中存在免疫细胞，是疫苗投递的一个极具潜力的优秀器官。然而，目前还没有简单且廉价的皮肤疫苗接种方法。本文研究了一种简单、廉价的微针给药装置，即经 DNA 涂层的微米级针。使用微针接种编码丙型肝炎病毒非结构 3/4A 蛋白的质粒，可有效刺激特异性细胞毒性 T 淋巴细胞（CTL）。重要的是，微创微针在刺激 CTL 方面与基因枪和皮下注射等更复杂或侵入性的给药技术一样有效。因此，微针可能为 DNA 疫苗接种提供一种有前景的技术。
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