氧化葡萄糖酸杆菌 ZJB09112 发酵甘油生产 1,3-二羟基丙酮。
Production of 1,3-dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112.
机构信息
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.
出版信息
J Microbiol Biotechnol. 2010 Feb;20(2):340-5.
The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Gluconobacter oxydans ZJB09112 in shake flask and bubble column bioreactor. After fermentation in the optimized medium (g/L: yeast extract 5, glycerol 2.5, mannitol 22.5, K(2)HPO(4) 0.5, KH(2)PO(4) 0.5, MgSO(4) x 7H(2)O 0.1, CaCO(3) 2.0, pH 5.0), when five times of glycerol feeding were made, 161.9 + or - 5.9 g/l of DHA was attained at 88.7 + or - 3.2% conversion rate of glycerol to DHA.
优化了文化变量，以提高在摇瓶和鼓泡塔生物反应器中氧化葡萄糖杆菌 ZJB09112 生产 1,3-二羟基丙酮（DHA）的产量。在优化的培养基（g/L：酵母提取物 5、甘油 2.5、甘露醇 22.5、K 2 HPO 4 0.5、KH 2 PO 4 0.5、MgSO 4 x 7H 2 O 0.1、CaCO 3 2.0、pH 5.0）中发酵时，当进行五次甘油进料时，达到了 161.9+/-5.9 g/L 的 DHA，甘油到 DHA 的转化率为 88.7+/-3.2%。
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